EC Blue Broth

EC Blue Brothis recommended for detection and confirmation of Escherichia coli and total coliforms from water samples, using a combination of chromogenic and fluorogenic substrates.Escherichia coli can be distinguished from other coliforms by its unique ability to fluoresce in the presence of fluorogenic substrate. The fluorogenic substrate, MUG is split by enzyme β-glucuronidase especially present in Escherichia coli. The reaction is indicated by the development of a blue fluorescence under UV light. The presence of total coliforms is indicated by blue-green colourations due to the cleavage of the chromogenic substrate (X-Gal). IPTG amplifies enzyme synthesis and increases the activity of β -galactosidase.