ECC Selective Medium (without Membrane Filter)

Recommended for the simultaneous detection of Escherichia coli and total coliforms in water and food based on chromogenic differentiation. It contains two chromogenic substrates. The enzyme ß-D-galactosidase produced by coliforms cleaves one of the chromogen to form salmon to red coloured colonies (5). The enzyme produced by E.coli, cleaves X-glucuronide, the other chromogen. Colonies of E.coli gives dark blue to violet coloured due to presence of ß-D-glucuronidase and ß-D-galactosidase. On addition, on Kovacs reagent, formation of cherry red colour indicates a positive indole reaction. Sterile pad is supplied in sterile Petri plate. Membrane filter is not supplied .