Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Brucella#CC293D
Intended Use
Recommended for the isolation and identification of Brucella species.
Composition**
| Ingredients | g/L |
|---|---|
| HM infusion B from 500g # | 10.000 |
| Tryptose | 10.000 |
| Sodium chloride | 5.000 |
| Gelatin | 1.000 |
| Dextrose (Glucose) | 2.500 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef heart, infusion from
Directions
Suspend 21.75 grams in 500 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add sterile 10% v/v Sheep blood. Also add rehydrated contents of one vial of NPBCVN Selective Supplement (FD005). Mix well and pour into sterile Petri plates.
Principle And Interpretation
Brucellosis is a zoonotic disease with a domestic animal reservoir. It is an occupational disease of veterinarians, microbiologists, farmers etc. The route of infections is genital, nasopharyngeal, gastrointestinal, conjunctival, respiratory and through abraded skin (1,2). Brucellosis in humans has a variable incubation period, an insidious or abrupt onset and no pathognomic symptoms or signs. Brucella Agar was designed for cultivating Brucella species from diagnostic specimens. With the incorporation of blood or other nutritious substances, it facilitates the cultivation of variety of fastidious anaerobic organisms (3). However, Brucella Medium is supplemented with antibiotics to prevent overgrowth of other accompanying organisms. Brucella Agar Base w/ 1.0 % Dextrose was originally developed by Jones and Morgan (4) for preparations of serum-dextrose-antibiotic medium used for the isolation and cultivation of Brucella species. The medium contains HM infusion B and tryptose, which facilitates cultivation of variety of fastidious anaerobic organisms; by providing essential nutrients. Gelatin serves as a source of nutrients. Glucose serves as source of energy. Addition of antibiotics (as FD) makes the medium highly selective for Brucella species. Ethyl violet and circulin, which were recommended initially, are no longer used (5).
Type of specimen
Clinical samples: faeces
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (6,7).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- All presumptive anaerobic organisms must be identified by confirmatory test
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light yellow coloured, clear to slightly opalescent gel forms in Petri plates On addition of 10% v/v sterile sheep blood cherry red coloured opalescent gel forms in Petri plates
Reaction
Reaction of 4.35% w/v aqueous solution at 25°C. pH: 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed in presence of 10% Carbon dioxide (CO2) atmosphere with added sterile 10%v/v sheep blood and NPBCVN Selective Supplement (FD005),after an incubation at 35-37°C for 24-48 hours
| Organism | Growth |
|---|---|
| Brucella melitensis ATCC 4309 | luxuriant |
| Brucella suis ATCC 4314 | luxuriant |
| Escherichia coli ATCC 25922 (00013*) | inhibited |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | inhibited |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).
| Product Name | Brucella Selective Medium Base |
|---|---|
| SKU | M822 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of ClinicalMicrobiology, ASM, Washington, D.C. |
| Customized Product Available | No |
Write Your Own Review
Brucella Agar Base
As low as
₹1,404.00
Brucella Agar Base w/ Hemin and Vitamin K
As low as
₹7,334.00
Brucella Vitamin K1 Blood Agar Base
As low as
₹6,425.00
Upsell Products
Brucella Agar Plate
As low as
₹1,210.00
Brucella Agar Plate with Hemin & Vitamin K1
As low as
₹2,679.00