Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for enrichment, isolation and cultivation of Brucella or Campylobacter species from clinical and non-clinical specimen
Composition**
| Ingredients | g/L |
|---|---|
| Tryptone | 10.000 |
| Peptone | 10.000 |
| Yeast extract | 2.000 |
| Dextrose (Glucose) | 1.000 |
| Sodium chloride | 5.000 |
| Sodium bisulphite | 0.100 |
| Horse serum | 50.000ml |
NPBCVN Selective Supplement (FD005). 2Vials
*Ingredients
| Ingredients | Concentration |
|---|---|
| Polymyxin B sulphate | 2500IU |
| Bacitracin | 12500IU |
| Nystatin | 50000IU |
| Cycloheximide | 50mg |
| Naildixic acid | 2.500mg |
| Vancomycin | 10mg |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Brucella are intracellular parasites that cause epizootic abortions in animals and septicemic febrile illness or localized infections of bone, tissue or organ systems in humans (1,2). Brucella species are highly fastidious and therefore require a nutrient rich medium to be able to grow. Also, Brucella species are highly infective and so extreme care should be taken while handling. Brucella Agar Base is used for the isolation and cultivation of Brucella species. The basal medium (with addition of Campylobacter Supplements) can be also used for the isolation of Campylobacter (3). Brucella Medium is a modified medium formulated to support luxuriant growth of fastidious bacteria like Brucella, streptococci, pneumococci, Listeria, Neisseria meningitides and Haemophilus influenzae (4). Brucella Agar is also recommended by APHA for isolation of Brucella species from foods (5).
Tryptone and peptone provide nitrogen and carbon source, long chain amino acids, vitamins and other essential nutrients Yeast extract serves as a source of vitamin B complex, and additionally it also supplies some nitrogenous nutrients. Sodium bisulphite is a reducing agent and sodium chloride helps to maintain the osmotic equilibrium of the medium. Dextrose serves as an energy source. The medium can also be enriched with 5% v/v sterile defibrinated horse blood. For selective isolation of Brucella species antibiotic mixtures in the form of freeze dried supplements (FD) are incorporated into the base (6,7,8).
Type of specimen
Clinical: Blood
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,9).
Swab specimens can be directly streaked on the plate. Liquid specimens can be inoculated by means of an inoculation loop.
When non-selective medium is required, Brucella Broth Base may be employed with the addition of serum only (i.e. without antibiotics).
Warning and Precautions
In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- All presumptive anaerobic organisms must be identified by confirmatory test.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Brucella Agar in 90mm disposable plate with smooth surface and absence of black particles/cracks/bubbles Colour of medium
Yellow coloured medium
Quantity of medium
25 ml of medium in 90 mm disposable plate
pH
6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-72 hours in presence of 10% CO2
| Organism | Inoculum (CFU) |
Growth |
|---|---|---|
| Brucella melitensis ATCC 4309 | 50-100 | luxuriant |
| Brucella suis ATCC 4314 | 50-100 | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=103 | inhibited |
| Escherichia coli ATCC 25922 (00013*) | >=103 | inhibited |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,9).
| Product Name | Brucella Agar Plate |
|---|---|
| SKU | MP074 |
| Customized Product Available | No |
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