Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Inoculum Preparation#CC293D
Composition
| Ingredients | Gms/Litre |
|---|---|
| Peptonized milk | 15.000 |
| Yeast extract | 5.000 |
| Dextrose | 10.000 |
| Monopotassium phosphate | 2.000 |
| Tomato juice (100 ml) | 5.000 |
| Polysorbate 80 | 1.000 |
| Final pH (at 25°C) | 6.8±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 38 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Distribute in tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Pantothenate Inoculum Broth is prepared based on the formula originally designed by Kulp and White (1) and later on modified and recommended by AOAC (2) for cultivating Lactobacilli used in microbiological assays. Kulp and White developed this medium for plating Lactobacillus acidophilus and obtained high recovery of Lactobacilli (1).
Peptonized milk contains lactose, which serves as the energy source for Lactobacilli. Dextrose serves as the fermentable carbohydrate and/or energy source. Yeast extract and peptone (of peptonized milk) provide vitamin B complex, nitrogenous compounds and trace ingredients for the growth. Tomato juice provides an acid environment in the medium resulting in inhibition of microorganisms other than acidophilic bacteria. Polysorbate 80 supplies fatty acids required for the metabolism of Lactobacilli.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium: Medium amber coloured clear solution, without any precipitate.
Reaction: Reaction of 3.8% w/v aqueous solution at 25°C. pH : 6.8±0.2
Cultural Response
M542: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Lactobacillus casei ATCC 9595 | 50-100 | luxuriant |
| Lactobacillus leichmannii ATCC 4797 | 50-100 | luxuriant |
| Lactobacillus plantarum ATCC 8014 | 50-100 | luxuriant |
| Lactobacillus acidophilus ATC 11506 | 50-100 | luxuriant |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Pantothenate Inoculum Broth |
|---|---|
| SKU | M542 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | Glass Bottle |
| References | 1. Kulp J. W. L. and White V., 1932, Science, 76:17. 2.Cunniff P., (Ed.), 1995, Official Methods of Analysis of AOAC International, 16th Ed., Vol. II, AOAC, Arlington, Virginia. |
| Customized Product Available | No |
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