Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for preparation of inoculum used in microbiological assays of Pantothenic acid or its salts.
Composition**
| Ingredients | Gms/Litre |
|---|---|
| HiVeg™ hydrolysate No. 3 | 15.000 |
| Yeast extract | 5.000 |
| Dextrose (Glucose) | 10.000 |
| Potassium dihydrogen phosphate | 2.000 |
| Tomato juice (100 ml) | 5.000 |
| Polysorbate 80 (Tween 80) | 1.000 |
| Final pH (at 25°C) | 6.8±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 38 grams in 1000 ml distilled water. Heat, if necessary to dissolve the medium completely. Dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Pantothenate Inoculum HiVeg Broth medium is prepared by using HiVeg hydrolysate No. 3 in place of Peptonized milk which is free from BSE/TSE risks associated with animal based peptones. This can be used for the same purpose of Pantothenate Inoculum Broth is prepared based on the formula originally designed by Kulp and White (1) and later on modified and recommended by AOAC (2) for cultivating Lactobacilli used in microbiological assays. This medium can also be used for plating Lactobacillus acidophilus and obtained high recovery of Lactobacilli.
HiVeg™ hydrolysate No. 3 serves as the energy source for Lactobacilli species. Dextrose serves as the fermentable carbohydrate and/or energy source. Yeast extract and HiVeg hydrolysate No.3 provides vitamin B complex, nitrogenous compounds and trace ingredients for the growth. Polysorbate 80 supplies fatty acids required for the metabolism of Lactobacilli. Tomato juice provides an acid environment in the medium resulting in inhibition of microorganisms other than acidophilic bacteria. Polysorbate 80 supplies fatty acids required for the metabolism of Lactobacilli.
Quality Control
Appearance: Cream to yellow may have green tinge homogeneous free flowing powder
Colour and Clarity of prepared medium: Medium amber coloured clear solution.
Reaction: Reaction of 3.8% w/v aqueous solution at 25°C. pH: 6.8±0.2
pH: 6.60-7.00
Cultural Response: Cultural characteristics observed after an incubation at 35 - 37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Lactobacillus casei ATCC 9595 | 50-100 | luxuriant |
| Lactobacillus leichmannii ATCC 4797 | 50-100 | luxuriant |
| Lactobacillus plantarum ATCC 8014 | 50-100 | luxuriant |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Pantothenate Inoculum HiVeg™ Broth |
|---|---|
| SKU | MV542 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | Glass Bottle |
| References | 1. Kulp J. W. L. and White V., 1932, Science, 76:17. 2.Cunniff P., (Ed.), 1995, Official Methods of Analysis of AOAC International, 16th Ed., Vol. II, AOAC, Arlington, Virginia. |
| Customized Product Available | No |
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