Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Special Media for Fungi#CC293D
Intended Use
Recommended for isolation, detection and cultivation of saprophytic fungi.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Malt extract | 40.000 |
| Sucrose | 30.000 |
| Sodium nitrate | 2.000 |
| Potassium chloride | 0.500 |
| Magnesium sulphate | 0.500 |
| Ferrous sulphate | 0.010 |
| Dipotassium hydrogen phosphate | 1.000 |
| Agar | 20.000 |
| Final pH (at 25°C) | 6.8±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 94.01 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Saprophytic fungi are the largest group of fungi, which grow on dead organic matter such as fallen trees, cow patties, dead leaves, and even dead insects and animals. These fungi have enzymes that work to "rot" or "digest” the cellulose and lignin found in the organic matter, with the lignin being an important source of carbon for many organisms. Penicillium, commonly known as "bread mould”, is a saprophytic fungus that has various industrial applications both in food and environment.
Czapek Malt Agar is used for isolation, detection and cultivation of saprophytic fungi, yeasts and moulds, mainly for Penicillium (1).
This medium contains sodium nitrate as the sole source of nitrogen. Sucrose and malt extract serves as the carbohydrate sources for the growing fungi. Various salts in the medium not only buffer the medium but also provide essential ions to the fungi. Slightly acidic pH of the medium favours the growth of saprophytic fungi.
Type of specimen
Organic matter samples.
Specimen Collection and Handling:
For organic matter samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(1) After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 2.0% Agar gel
Colour and Clarity of preparred mediun
Medium amber coloured, slightly opalescent gel forms in Petri plates
Reaction
Reaction of 9.4% w/v aqueous solution at 25°C. pH: 6.8±0.2
pH
6.60-7.00
Cultural Response
Cultural characteristics observed after an incubation at 25-30°C for 48-72 hours
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| # Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | good-luxuriant | |
| Candida albicans ATCC 10231 (00054*) | 50-100 | good-luxuriant | >=70% |
| Saccharomyces cerevisiae ATCC 9763 (00058*) | 50-100 | good-luxuriant | >=70% |
| Pencillium notatum ATCC 10108 | 50-100 | luxuriant |
Key: *Corresponding WDCM numbers.
# - Formerly known as Aspergillus niger
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Use before expiry date on the label
Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | Czapek Malt Agar |
|---|---|
| SKU | M732 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Booth C., (Ed.), 1971, Methods in Microbiology by Norris J.R. and Ribbons, D.W., Vol. 4, Academic Press, London. 2.Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition. 3.Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)3Manual of Clinical Microbiology, 11th Edition. Vol. 1. |
| Customized Product Available | No |
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