Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Manganese Bacteria#CC293D
Composition**
Ingredients
| Ingredients | Gms / Litre |
|---|---|
| Beef extract | 1.000 |
| Yeast extract | 0.075 |
| Manganous carbonate | 2.000 |
| Ferrous ammonium sulphate | 0.150 |
| Sodium citrate | 0.150 |
| Agar | 12.000 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 15.37 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50-55°C and aseptically add filter-sterilized solution of cyanocobalamin to a final concentration of 0.005 mg/litre. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Leptothrix is a sheathed filamentous bacterium that can generally be found in different types of aquatic environments with sufficient organic matter. Leptothrix bacteria are known to be capable of oxidizing both iron (II) and manganese (II), unlike other sheathed bacteria. These belong to the group of nuisance organisms which have the ability to transform or deposit significant amount of iron, usually in the form of objectionable slimes. Iron bacteria (Leptothrix) may cause, or be associated with, fouling and plugging of wells. They also cause odour, taste, frothing, colour and increases turbidity in waters.
Mn Agar is formulated in accordance with APHA (1) and is used as a differential medium (2) based on the ability of Leptothrix species to oxidize manganous ion.
Beef extract and yeast extract supply the essential growth nutrients. Leptothrix-Sphaerotilus derive energy by oxidation of ferrous sulphate. Alternatively Leptothrix may be grown by direct plating on Mn Agar No. 2 (3).
Quality Control
Appearance
White to cream homogeneous free flowing powder
Gelling
Firm, comparable with 1.2% Agar gel.
Colour and Clarity of prepared medium
Yellow coloured clear to slightly opalescent gel with a slight precipitate forms in Petri plates
Cultural Response
M771: Cultural characteristics observed after an incubation at 25-30°C for 24-48 hours.
| Organism | Growth | Manganese oxidation |
|---|---|---|
| Leptothrix discophora ATCC 43182 | luxuriant | positive reaction |
| Sphaerotilus natans ATCC 13338 | good | negative reaction |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expirydate on the label.
| Product Name | Mn Agar Base |
|---|---|
| SKU | M771 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Eaton A. D., Clesceri L. S., Rice E. W. and Greenberg A W., (Eds.),2005, Standard Methods for the Examination of Waterand Wastewater, 21st Ed., APHA, Washington, D.C. 2.Mulder E. G. and VanVeen W. L., 1963, Antonie Van Leeuwenhock (Holland), 29:121. 3.Ghiorse W. C., 1984, Ann. Rev. Microbiol., 38:515. |
| Customized Product Available | No |
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