Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Mn HiVeg Agar Base is used for detection of Leptothrix species by its ability to oxidize manganous ion.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg extract | 1.0 |
| Yeast extract | 0.075 |
| Manganous carbonate | 2.0 |
| Ferrous ammonium sulphate | 0.15 |
| Sodium citrate | 0.15 |
| Agar | 12.0 |
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 15.4 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50-55°C and aseptically add filter sterilized solution of Cyanocobalamin to a final concentration of 0.005 mg/litre.
Principle and Interpretation
This medium is prepared by replacing Beef extract with HiVeg extract, that makes the medium free of BSE/TSE risks. Mn HiVeg Agar Base is the modification of Mn Agar Base which is formulated in accordance with APHA (1) and is used as a differential medium (2) based on the ability of Leptothrix species to oxidize manganous ion. HiVeg extract and yeast extract supply the essential growth nutrients. Leptothrix-Sphaerotilus derive energy by oxidation of ferrous sulphate.
Quality Control
Appearance of powder: Cream coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Technical Data
Product Profile
| Vegetable based (Code MV) | MV771, HiVeg extract |
|---|---|
| Animal based (Code M) | M771, Beef extract |
| Recommended for | Detection of Leptothrix species by its ability to oxidize manganous ion. |
| Reconstitution | 15.4 g/l |
|---|---|
| Quantity on preparation (500g) | 32.46 L |
| pH (25°C) | - |
| Supplement | Cyanocobalamin |
| Sterilization | 121°C / 15 minutes. |
| Storage | Dry Medium - Below 30°C, Prepared Medium 2-8°C. |
Gelling: Firm, comparable with 1.2% Agar gel.
Colour and Clarity: Yellow coloured, clear to slightly opalescent gel forms with slight precipitate in petri plates.
Cultural Response: Cultural characteristics observed after an incubation at 25°C for 24 - 48 hours.
Organisms (ATCC)
| Growth | Manganous oxidation | |
|---|---|---|
| Leptothrix (Sphaerotilus discophorous) | luxuriant | + |
| Sphaerotilus natans (13338) | good | - |
| Product Name | Mn HiVeg™ Agar Base |
|---|---|
| SKU | MV771 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Eaton A. D., Clesceri L. S., Rice E. W. and Greenberg A W., (Eds.),2005, Standard Methods for the Examination of Waterand Wastewater, 21st Ed., APHA, Washington, D.C. 2.Mulder E. G. and VanVeen W. L., 1963, Antonie Van Leeuwenhock (Holland), 29:121. 3.Ghiorse W. C., 1984, Ann. Rev. Microbiol., 38:515. |
| Customized Product Available | No |
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