Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Vibrio species#CC293D
Intended Use
Used for isolation of Vibrio parahaemolyticus.
Composition
| Ingredients | g/L |
|---|---|
| Peptone | 10.000 |
| Yeast extract | 5.000 |
| Sodium taurocholate | 5.000 |
| Sodium thiosulphate | 10.000 |
| Sodium chloride | 20.000 |
| Sodium lauryl sulphate (SLS) | 0.200 |
| Sodium citrate | 10.000 |
| Sucrose | 20.000 |
| Bromo thymol blue | 0.040 |
| Thymol blue | 0.040 |
| Agar | 20.000 |
| Final pH (at 25°C) | 8.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 100.28 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Vibrios's are short, often curved, gram-negative rods that are motile by means of a single polar sheathed flagellum. Their growth is stimulated by Na+ ions, which is an absolute requirement for most species. Vibrio parahaemolyticus, a halophilic Vibrio, is responsible worldwide for outbreaks of gastroenteritis associated with eating many kinds of contaminated sea foods. It has been isolated from raw shellfish and other fish in the warm coastal and estuarine waters (1).
VP Medium is prepared according to formula of De et al (2) and is recommended for selective isolation of Vibrio species, especially V. parahaemolyticus from clinical specimens, foodstuffs, and environmental sample (3). The medium contains peptone and yeast extract, which provide nitrogenous compounds, vitamin B complex and other essential growth nutrients. Sucrose is added as a fermentable sugar. Sodium citrate, sodium lauryl sulphate, sodium taurocholate and sodium thiosulphate as well as high alkalinity of the medium inhibit most of the contaminating organisms. Bromothymol blue and thymol blue are the pH indicators. The alkaline pH of the medium and higher concentration of sodium chloride improves the recovery of Vibrio parahaemolyticus. Sucrose fermenting organisms like V. cholerae and V. alginolyticus produces yellow coloured colonies. Vibrio parahaemolyticus is a sucrose non-fermenting organism and produces blue-green colonies, as does V. vulnificus.
Type of specimen
Clinical samples - faeces samples; Food samples
Specimen Collection and Handling:
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5).
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (6).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
In Vitro diagnostic Use. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Occasionally a few enteric sucrose non-fermenters may exhibit growth e.g. Proteus group (5).
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to greenish yellow homogeneous free flowing powder
Gelling
Firm, comparable with 2.0% Agar gel.
Colour and Clarity of prepared medium
Bluish coloured clear to slightly opalescent gel forms in Petri plates.
Reaction
Reaction of 10.0% w/v aqueous solution at 25°C. pH : 8.6±0.2
pH
8.40-8.80
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of colony |
|---|---|---|---|---|
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | poor | <=10% | yellow |
| Escherichia coli ATCC 25922 (00013*) | >=104 | inhibited | 0% | |
| Shigella flexneri ATCC 12022 (00126*) | >=104 | inhibited | 0% | |
| Vibrio cholerae ATCC 15748 | 50-100 | good-luxuriant | >=50% | yellow |
| Vibrio parahaemolyticus ATCC 17802 (00037*) | 50-100 | good-luxuriant | >=50% | bluish-green |
| Vibrio vulnificus ATCC 27562 | 50-100 | good-luxuriant | >=50% | greenish yellow |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | VP Medium |
|---|---|
| SKU | M662 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Collee J. G., Fraser A. G., Marmion B. P., Simmons A., (Eds.), Mackie and McCartney, Practical Medical Microbiology,1996, 14th Edition, Churchill Livingstone2.De S. P., Sen P., De C., Ghosh A., Pal S. C., 1977, Indian J. Med. Res. 66,398. 3.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol. 1, Williams and Wilkins, Baltimore. |
| Customized Product Available | No |
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