Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Lactobacilli#CC293D
Intended Use
Used for the cultivation and enumeration of Lactobacilli from saliva and other acidophilic bacteria.
Composition
| Ingredients | g/L |
|---|---|
| Tomato juice (400 ml) | 20.000 |
| Peptone | 10.000 |
| Peptonized SM powder# | 10.000 |
| Agar | 20.000 |
| Final pH (at 25°C) | 5.0±0.2 |
Formula adjusted, standardized to suit performance parameters
# Equivalent to Peptonized milk
Directions
Suspend 60.0 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Lactic acid bacteria are acid-tolerant, non-sporulating rods or cocci widely distributed in nature and historically linked to food fermentation. Lactobacilli form the normal flora of the human mouth, intestinal tract and vagina and may therefore be recovered from pathological specimens as contaminants (1). Tomato juice was included in media for lactobacilli (2) and was found to be advantageous for its growth, particularly Lactobacillus acidophilus (3).
Tomato Juice Agar, Special is formulated as per Jay (4,5) for the direct plate count of lactobacilli and other acidophilic bacteria, especially from saliva (6). Tomato Juice Agar, Special is similar to Tomato Juice Agar, except that the agar concentration is increased to 20 grams per liter and the pH is adjusted to 5.0 in the former.
Tomato juice provides an acid environment and is also a source of carbon, and other essential nutrients. Peptonized SM powder provides lactose, which acts as the energy source. Peptone provides nitrogenous, carbonaceous compounds, trace elements and other essential growth nutrients. The low pH of medium inhibits many commensal bacteria and encourages growth of Lactobacilli. Tomato Juice Agar, Special is more selective than Tomato Juice Agar (6).
Type of specimen
Clinical samples - Saliva
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (7,8).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic Use. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further biochemical and serological tests must be carried out for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 2.0% Agar gel.
Colour and Clarity of prepared medium: Medium amber coloured clear to slightly opalescent gel forms in Petri plates.
Reaction: Reaction of 6.0% w/v aqueous solution at 25°C. pH: 5.0±0.2
pH: 4.80-5.20
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Lactobacillus acidophilus ATCC 4356 (00098*) | 50-100 | luxuriant | >=50% |
| Lactobacillus casei ATCC 9595 | 50-100 | luxuriant | >=50% |
| Lactobacillus leichmannii ATCC 4797 | 50-100 | luxuriant | >=50% |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited | 0% |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | Tomato Juice Agar, Special |
|---|---|
| SKU | M879 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,8th Ed., American Society for Microbiology, Washington, D.C.2.Mickle F. L. and Breed R. S.,1925, Technical Bulletin 110, N.Y. State Agriculture Exp. Station, Geneva, N.Y.3.Kulp W. L., 1927, Science 66:512. |
| Customized Product Available | No |
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