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Lactic Streptococci#CC293D
Intended Use
Sucrose Agar is recommended isolation of dextran producing by Leuconostoc species.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Casein enzymic hydrolysate | 10.000 |
| Yeast Extract | 5.000 |
| Dipotassium phosphate | 5.000 |
| Triammonium phosphate | 5.000 |
| Sucrose | 50.000 |
| Agar | 15.000 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 9 grams in 100 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 25°C and store in a cool, dry place preferably below 25°C.
Principle And Interpretation
Sucrose Agar is modification of the medium developed by Boatwright and Kirsop for brewery isolates (1). Leuconostoc are fastidious chemoorganotrophic bacteria. Casein enzymic hydrolysate and yeast extract provides as nitrogen source and other essential nutrients. The phosphates act as buffering system. Leuconostoc spp synthesizes dextran from sucrose.
Quality Control
Appearance: Cream to yellow coloured homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Light amber coloured clear to slightly opalescent gel forms in petri plates.
Cultural Response: M828: Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Growth |
|---|---|
| Leuconostoc dextranicum | good-luxuriant |
| Leuconostoc mesenteroides | good-luxuriant |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Sucrose Agar for Brewery Isolates |
|---|---|
| SKU | M828 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Boatwright J. and Kirsop B.H., 197 |
| Customized Product Available | No |
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