Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Sucrose HiVeg Agar is recommended for isolation of dextran producing Leuconostoc species.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate | 10.0 |
| Yeast extract | 5.0 |
| Dipotassium phosphate | 5.0 |
| Triammonium phosphate | 5.0 |
| Sucrose | 50.0 |
| Agar | 15.0 |
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 90.00 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 25°C and store in a cool, dry place preferably below 25°C.
Principle and Interpretation
Sucrose HiVeg Agar is prepared by using HiVeg hydrolysate in place of Casein enzymic hydrolysate making the medium free from BSE/TSE risks. Sucrose HiVeg Agar is modification of the medium developed by Boatwright and Kirsop for brewery isolates(1). Leuconostoc are fastidious chemo-organotrophic bacteria. HiVeg hydrolysate and yeast extract seves as nitrogen source and other essential nutrients. The phosphates act as buffering system. Leuconostoc species synthesizes dextran from sucrose.
Quality Control
Appearance of powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Product Information
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV828 | M828 |
| HiVeg hydrolysate | Casein enzymic hydrolysate |
Recommended for : Detection of dextran producing Leuconostoc species.
Preparation Details
- Reconstitution : 30.15 g/l
- Quantity on preparation (500g) : 16.58 L
- pH (25°C) : -
- Supplement : None
- Sterilization : 121°C / 15 minutes.
- Storage : Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Physical Characteristics
- Gelling : Firm, comparable with 1.5% Agar gel.
- Colour and Clarity : Light amber coloured, clear to slightly opalescent gel forms in petri plates.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18 - 48 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth |
|---|---|---|
| Leuconostoc dextranicum (17079) | 102-103 | good-luxuriant |
| Leuconostoc mesenteroides (12291) | 102-103 | good-luxuriant |
| Product Name | Sucrose HiVeg™ Agar for Brewery Isolates |
|---|---|
| SKU | MV828 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Boatwright J. and Kirsop B.H., 197 |
| Customized Product Available | No |
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