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Blood Free Campylobacter Selectivity HiVeg™ Agar Base
As low as
$256.30
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV887
Recommended for selective isolation and differentiation of Campylobacter species from food and animal feeding stuffs.
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Intended Use
Blood Free Campylobacter Selectivity HiVeg Agar Base is used for selective isolation and differentiation of Campylobacter species.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg extract | 10.0 |
| HiVeg peptone | 10.0 |
| HiVeg hydrolysate | 3.0 |
| Sodium chloride | 5.0 |
| Synthetic detergent No. III | 1.0 |
| Ferrous sulphate | 0.25 |
| Sodium pyruvate | 0.25 |
| Charcoal, bacteriological | 4.0 |
| Agar | 12.0 |
Final pH (at 25°C) 7.4 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Product Profile
Vegetable based (Code MV) MV887
- HiVeg hydrolysate
- HiVeg peptone
- HiVeg extract
- Synthetic detergent No. III
Animal based (Code M) M887
- Casein enzymic hydrolysate
- Peptic digest of animal tissue
- Beef extract
- Sodium deoxycholate
Recommended for: Selective isolation and differentiation of Campylobacter species.
Reconstitution
Quantity on preparation (500g): 45.5 g/l
pH (25°C): 7.4 ± 0.2
Supplement: Campylobacter Supplement V (FD067) / CAT Selective Supplement (FD145)
Sterilization
121°C / 15 minutes.
Storage
Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Directions
Suspend 22.75 grams in 500 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add rehydrated contents of one vial of Campylobacter Supplement V (FD067). Alternatively to increase the selectivity of the medium, rehydrated contents of one vial of CAT Selective Supplement (FD145) may be added to 500 ml sterile molten base. Mix well and pour into sterile petri plates.
Principle and Interpretation
This medium is prepared by completely replacing animal based peptones by vegetables peptones. The performance of this medium is equivalent to conventional CCDA-Preston Blood Free Medium cited in APHA (1) for selective isolation of Campylobacter jejuni, Campylobacter coli, Nalidixic acid resistant, thermophilic Campylobacter species and Campylobacter laridis from clinical specimens. Blood is replaced by charcoal. Ferrous sulphate and sodium pyruvate enhance aerotolerance of Campylobacter species. Campylobacter isolation depends on the antimicrobial agents in the medium, a microaerophilic environment and the incubation temperature. Incubation at 42°C under microaerobic conditions yields maximum growth of Campylobacter. Colonies tend to swarm when initially isolated from clinical specimens.
Gelling
Firm, comparable with 1.2% Agar gel.
Colour and Clarity
Black coloured, opaque gel forms in petri plates.
Reaction
Reaction of 4.55% w/v aqueous solution is pH 7.4 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 42°C for 24 - 48 hours on addition of Campylobacter Supplement V (FD067).
| Organisms (ATCC) | Growth | Colour of colony |
|---|---|---|
| Campylobacter coli (33559) | good - luxuriant | Creamy grey |
| Campylobacter jejuni (29428) | good - luxuriant | Grey |
| Campylobacter laridis | good - luxuriant | Varying type |
| Escherichia coli (25922) | inhibited | - |
Quality Control
Appearance of powder
Grey coloured, homogeneous, free flowing powder.
| Product Name | Blood Free Campylobacter Selectivity HiVeg™ Agar Base |
|---|---|
| SKU | MV887 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Vanderzant C. and Splittstoesser D. (Ed.), 1992, Compendium of Methods ForMicrobiological Examination of Food, 3rd ed., APHA, Washington, D.C. |
| Customized Product Available | No |
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