Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Lactobacillus MRS HiVeg™ Broth (MRS HiVeg™ Broth)
As low as
$51.70
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV369
For cultivation of all Lactobacilli.
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Intended Use
Recommended for cultivation of all Lactobacilli.
Composition**
| Ingredients | g/L |
|---|---|
| HiVeg® peptone No. 3 | 10.000 |
| HiVeg®extract | 10.000 |
| Yeast extract | 5.000 |
| Dextrose(Glucose) | 20.000 |
| Polysorbate 80 (Tween 80) | 1.000 |
| Ammonium citrate | 2.000 |
| Sodium acetate | 5.000 |
| Magnesium sulphate | 0.100 |
| Manganese sulphate | 0.050 |
| Dipotassium hydrogen phosphate | 2.000 |
| Final pH (at 25°C) | 6.5±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 55.15 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Distribute in tubes, bottles or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Lactobacilli MRS media are based on the formulation of deMan, Rogosa and Sharpe (1) with slight modification. It supports luxuriant growth of all Lactobacilli from oral cavity (2), dairy products (3), foods (2), faeces (4,5) and other sources (6).
Lactobacillus MRS HiVeg® Broth (MRS HiVeg® Broth) is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. HiVeg® peptone No. 3 and HiVeg® extract supply nitrogenous and carbonaceous compounds. Yeast extract provides vitamin B complex and dextrose is the fermentable carbohydrate and energy source. Polysorbate 80 supplies fatty acids required for the metabolism of Lactobacilli. Sodium acetate and ammonium citrate inhibit Streptococci, moulds and many other microorganisms.
Type of specimen
Food and dairy samples
Specimen Collection and Handling:
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (2,7,8). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Medium amber coloured, clear to slightly opalescent solution in tubes
Reaction
Reaction of 5.51% w/v aqueous solution at 25°C. pH: 6.5±0.2
pH
6.30-6.70
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours or longer. (with 5% CO2)
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Lactobacillus fermentum ATCC 9338 | 50-100 | luxuriant |
| Lactobacillus leichmannii ATCC 7830 | 50-100 | luxuriant |
| #Lactiplantibacillus plantarum ATCC 8014 | 50-100 | luxuriant |
| Lactobacillus casei ATCC 9595 | 50-100 | luxuriant |
| Lactobacillus saki ATCC 15521 (00015*) | 50-100 | luxuriant |
| Lactobacillus lactis ATCC 19435 (00016*) | 50-100 | luxuriant |
| Pediococcus pentosaceas ATCC 33316 (00158*) | 50-100 | luxuriant |
Key: * Corresponding WDCM numbers. #Formerly known as Lactobacillus plantarum
Storage and Shelf Life
Store dehydrated and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | Lactobacillus MRS HiVeg™ Broth (MRS HiVeg™ Broth) |
|---|---|
| SKU | MV369 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. deMan J., Rogosa M. and Sharpe M., 1960, J. Appl. Bacteriol., 23:130. 2.Marshall R.T. (Ed.), 1992, Standard Methods for the Examination of Dairy Products, 16th ed., APHA, Washington,D.C. 3.Downes F.P.and Ito K.,(Eds.),2001,Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., APHA,Washington, D.C. 4.Sabine and Vaselekos, 1965, Nature, 206:960. 5.MacFaddin J., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol.1, Williams andWilkins, Baltimore. |
| Customized Product Available | No |
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