Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
Your enquiry has been submitted
Something problem with the server. Try again.
Glucose Yeast Extract Acetate Broth
As low as
$236.50
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
M964
Recommended for cultivation of Lactobacilli.
Your enquiry has been submitted
Please login to Post an Enquiry.
Intended Use
Recommended for cultivation of Lactobacilli
Composition
| Ingredients | g/L |
|---|---|
| Sodium acetate | 10.000 |
| Peptone | 10.000 |
| Yeast extract | 10.000 |
| Dextrose (Glucose) | 10.000 |
| Dipotassium hydrogen phosphate | 0.250 |
| Potassium dihydrogen phosphate | 0.250 |
| Magnesium sulphate | 0.100 |
| Ferrous sulphate | 0.005 |
| Manganese sulphate | 0.005 |
| Sodium chloride | 0.005 |
| Final pH (at 25°C) | 6.9±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 40.61 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Glucose Yeast Extract Acetate Broth is prepared by slight modification of the formula described by Evans and Niven (1) and Rogosa et al (2) and is used for enumerating Lactobacilli in pharmaceutical preparations.
It contains a variety of salts like sulphates, phosphates to support the growth of Lactobacilli. Necessary nitrogenous nutrients for Lactobacilli are provided by peptone and yeast extract. Glucose is the source of fermentable carbohydrate. The metallic salts are sources of ions essential for the replication of lactic acid bacteria. Sodium acetate inhibits Streptococci, moulds and many other organisms.
Type of specimen
Food and dairy samples; Pharmaceutical samples
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (3,4, 5). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Further serological and biochemical testing is required for complete identigication.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within theexpiry period when stored at recommended temperature.
Quality Control
Appearance: Cream to yellow coloured homogeneous free flowing powder
Colour and Clarity of prepared medium: Light yellow coloured clear solution without any precipitate.
Reaction: Reaction of 4.06% w/v aqueous solution at 25°C. pH: 6.9±0.2
pH: 6.70-7.10
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 24 - 48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Lactobacillus acidophilus ATCC 4356 (00098*) | 50-100 | luxuriant |
| Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 (00102*) | 50-100 | luxuriant |
| #Lactobacillus rhamnosus ATCC 9595 | 50-100 | luxuriant |
| ! Lactobacillus leichmannii ATCC 7830 | 50-100 | luxuriant |
| Lactobacillus plantarum ATCC 8014 | 50-100 | luxuriant |
Key: * Corresponding WDCM numbers, # Formerly known as Lactobacillus casei, ! Formerly known as Lactobacillus leichmannii
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).
References
- Evans and Niven, 1951, J. Bact., 62:599.
- Rogosa M., Mitchell J.A. and Wiseman R.F., 1951, J. Bact., 62:132.
- American Public Health Association, Standard Methods for the Examination of Dairy Products, Ed., Washington D.C.
- Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed., APHA Inc., Washington, D.C.
- Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed., American Public Health Association, Washington, D.C.
- Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.
- Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
| Product Name | Glucose Yeast Extract Acetate Broth |
|---|---|
| SKU | M964 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Evans and Niven, 1951, J. Bact., 62:599.2.Rogosa M., Mitchell J.A. and Wiseman R.F., 1951, J. Bact., 62:132. |
| Customized Product Available | No |
Write Your Own Review
M17 Agar w/o Lactose
As low as
$588.50
Lactic Agar
As low as
$79.20
M16 Agar (Modified Rogosa Agar)
As low as
$238.70
Streptococcus lactis Differential Agar Base
As low as
$232.10
Lactic Streak Agar
As low as
$270.60
M17 Agar Base
As low as
$232.10
Yeast Glucose Beef Broth (Revised as Yeast Glucose HM Peptone B Broth)
As low as
$256.30
Neutral Red Chalk Lactose Agar
As low as
$236.50
Upsell Products
Orange Serum Broth
As low as
$146.30
Tomato Juice Medium (without Membrane Filter)
As low as
$136.40