Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Syncase Broth
As low as
$254.10
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
M949
For the detection of coliforms in food samples.
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Composition
| Ingredients | Gms/Litre |
|---|---|
| Casein acid hydrolysate | 20.000 |
| Dipotassium phosphate | 8.710 |
| Yeast extract | 6.000 |
| Sodium chloride | 2.500 |
| Final pH (at 25°C) | 8.5±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 37.21 grams in 1000 ml distilled water. Heat, if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.
Note: The medium may form slight precipitate upon standing.
Principle And Interpretation
Examination of water, foods, ingredients and raw materials, for the presence of marker groups such as coliforms is one of the most common tests in a microbiological laboratory, partly because of the relative ease and speed with which these tests can be accomplished. It is a valuable bacterial indicator for determining the extent of faecal contamination of recreational surface waters or drinking water (1). Syncase Broth is used for the detection of coliform organisms from food samples as per APHA (2).
Casein acid hydrolysate and yeast extract in the medium supply the necessary nitrogenous nutrients for the growth of coliforms. Dipotassium phosphate serves as buffering agent to maintain pH, whereas sodium chloride maintains osmotic equilibrium.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium: Light yellow coloured clear solution in tubes
Reaction: Reaction of 3.7% w/v aqueous solution at 25°C. pH : 8.5±0.2
pH: 8.30-8.70
Cultural Response
M949: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Citrobacter freundii ATCC 8090 | 50-100 | luxuriant |
| Enterobacter aerogenes ATCC 13048 | 50-100 | luxuriant |
| Escherichia coli ATCC 25922 | 50-100 | luxuriant |
Storage and Shelf Life
Store below 30°Cin tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
Reference
- Corry J. E. L., Curtis G. D. W., and Baird R. M., Culture Media for Food Microbiology, Vol. 34, Progress in Industrial Microbiology, 1995, Elsevier, Amsterdam
- Vanderzant C. and Splittstoesser D. F., (Eds.), 1992, Compendium of Methods for the Microbiological Examination of Foods, 3rd Ed., APHA, Washington, D.C.
| Product Name | Syncase Broth |
|---|---|
| SKU | M949 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Corry J. E. L., Curtis G. D. W., and Baird R. M., Culture Media for Food Microbiology, Vol. 34, Progress in IndustrialMicrobiology, 1995, Elsevier, Amsterdam |
| Customized Product Available | No |
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