Nitrofurantoin Broth Base is used for the selective enrichment and isolation of Pseudomonas species. This medium contains Nitrofurantoin, 1-[(5-nitrofurfurylidene) amino] hydantoin, a synthetic antibacterial agent which is effective against most common gram-negative and gram-positive urinary tract pathogenic bacteria. Nitrofurantoin is not active against most strains of Proteus species or Serratia species. It has no activity against Pseudomonas species. Therefore nitrofurantoin incorporated in medium can be used as a selective medium for culturing of Pseudomonas species.
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