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Peptonized SM Agar
Fungi Yeasts and Molds#CC293D
Intended Use
For cultivation of lactic acid bacteria and examination of dairy products.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptonized milk | 15.000 |
| Agar | 12.000 |
| Final pH (at 25°C) | 6.5±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 27.0 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes and pour into sterile Petri plates.
Principle And Interpretation
Peptonized Milk Agar is used for the detection of lactic acid bacteria.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.2% Agar gel.
Colour and Clarity of prepared medium
Yellow coloured clear to slightly opalescent gel forms in Petri plates.
Reaction
Reaction of 2.7% w/v aqueous solution at 25°C. pH: 6.5±0.2
pH
6.30-6.70
Cultural Response
M442: Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 48 hours.
| Organism | Growth |
|---|---|
| Lactobacillus casei ATCC 7469 | luxuriant |
| Lactobacillus lactis ATCC 8000 | luxuriant |
| Lactobacillus leichmannii ATCC 7830 | luxuriant |
Storage and Shelf Life
Store below 30°C in tightly closed container. Use before expiry period on the label.
| Product Name | Peptonized SM Agar |
|---|---|
| SKU | M442 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| Customized Product Available | No |











