Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 5.0 |
| HiVeg extract | 3.0 |
| Lactose | 10.0 |
| Sodium chloride | 5.0 |
| China blue | 0.3 |
| Agar | 15.0 |
Final pH (at 25°C) 7.0 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) | ||
|---|---|---|---|
| MV580 | M580 | ||
| HiVeg peptone | Peptic digest of animal tissue | ||
| HiVeg extract | Beef extract | ||
| Recommended for | Differentiation and enumeration of bacteria in milk. | ||
| Reconstitution | 38.3 g/l | ||
| Quantity on preparation (500g) | 13.05 L | ||
| pH (25°C) | 7.0 ± 0.2 | ||
| Supplement | None | ||
| Sterilization | 121°C / 15 minutes. | ||
| Storage | Dry Medium - Below 30°C, Prepared Medium 2 - 8°C. | ||
Directions
Suspend 38.3 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
The medium is prepared by using HiVeg peptone and HiVeg extract which are free from BSE/TSE risks. China Blue Lactose HiVeg Agar is the modification of China Blue Lactose Agar, which was first designed by Brandl and Sobeck-Skal (1). It is a standard, non-inhibitory medium used for the differentiation and enumeration of bacteria in milk. China blue is a pH indicator which helps in differentiating lactose fermenters and lactose non-fermenters. However, this medium does not restrict the growth of cocci and hence can be used for the detection of Streptococci and Staphylococci as well. China Blue Lactose HiVeg Agar plates can be either streak inoculated directly or used for the pour-plate technique. Decimally diluted milk can be added to the molten, cooled medium.
Quality Control
Appearance of powder
Greenish yellow coloured, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and Clarity
Light blue coloured, slightly opalescent gel forms in petri plates.
Reaction
Reaction of 3.83% w/v aqueous solution is pH 7.0 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 22 - 24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery | Colour of colony |
|---|---|---|---|---|
| Enterococcus faecalis (29212) | 102-103 | luxuriant | >70% | blue |
| Escherichia coli (25922) | 102-103 | luxuriant | >70% | blue |
| Proteus vulgaris (13315) | 102-103 | luxuriant | >70% | colourless |
| Salmonella serotype Typhi (6539) | 102-103 | luxuriant | >70% | colourless |
| Shigella flexneri (12022) | 102-103 | luxuriant | >70% | colourless |
| Staphylococcus aureus (25923) | 102-103 | luxuriant | >70% | colourless |
MV580 China Blue Lactose HiVeg Agar
- Control
- Escherichia coli
- Enterococcus faecalis
- Staphylococcus aureus
| Product Name | China Blue Lactose HiVeg™ Agar |
|---|---|
| SKU | MV580 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Thomas S. B., 1974, the Microflora of Bulk Collected Milk- Part 2, Dairy Ind. Int. 39 (8): 279 2.De Vris T. 1975, Neth. Milk Dairy J., 29:127 3.Cousin M. A., 1982, J. Food Prot., 45:172 4.Brandl E. and Sobeck - Skal E., 1963, Milchwiss. Ber., 13:1. |
| Customized Product Available | No |
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