Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate | 15.00 |
| Papaic digest of soyabean meal | 5.00 |
| Sodium chloride | 5.00 |
| Lecithin | 0.70 |
| Polysorbate 80 (Tween 80) | 5.00 |
| Agar | 15.00 |
| Final pH (at 25°C) | 7.3 ± 0.2 |
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 45.7 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 12 to 15 lbs pressure (118 - 121°C) for 15 minutes.
Principle and Interpretation
Tryptone Soya HiVeg Agar with Lecithin and polysorbate 80 is prepared by replacing animal based peptones with vegetable peptones which makes the medium free of BSE/TSE risks. This medium is the modification of Tryptone Soya Agar with Lecithin and polysorbate 80 which is used in RODAC (Replicate Organism Detection and Counting) plates (1) for the detection and enumeration of microorganisms present on surfaces of sanitary importances (2, 3). HiVeg hydrolysate and Papaic digest of soyabean meal provide nitrogenous compounds and other nutrients essential for microbial replication. Lecithin and polysorbate 80 are neutralizers reported to inactivate residual disinfectants from where the sample is collected (4). Lecithin neutralizes quaternary ammonium compounds and polysorbate 80 neutralizes phenolic disinfectants, hexachlorophene, formalin and with lecithin ethanol (5). Collection of samples from areas before and after the treatment with disinfectant evaluates cleaning procedures in environmental sanitation. The presence and number of microorganisms is determined by the appearance of colonies on the agar surface (6). After counting the colonies, carry out biochemical testing for identification.
Quality Control
Appearance of Powder: Light yellow coloured, homogeneous, free flowing powder.
Gelling: Firm, comparable with 1.5% Agar gel.
Colour and Clarity: Light to medium amber coloured, slightly opalescent gel forms in petri plates.
Reaction: Reaction of 4.57% w/v aqueous solution is pH 7.3 ± 0.2 at 25°C.
Product Profile
Vegetable based (Code MV)
MV449
HiVeg hydrolysate
Animal based (Code M)
M449
Casein enzymic hydrolysate
Recommended for
Determining efficiency of sanitization of containers, equipment surfaces, water miscible cosmetics etc.
Preparation Details
Reconstitution: 45.7 g/l
Quantity on preparation (500g): 10.94 L
Quantity on preparation (100g): 2.18 L
pH (25°C): 7.3 ± 0.2
Supplement: None
Sterilization: 118°C-121°C / 15 minutes.
Storage: Dry Medium and Prepared Medium 2-8°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18 - 24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery | Colour of colony |
|---|---|---|---|---|
| Staphylococcus aureus (25923) | 102-103 | luxuriant | >70% | yellow to gold |
| Pseudomonas aeruginosa (27853) | 102-103 | luxuriant | >70% | yellow green |
MV449 Tryptone Soya HiVeg Agar w/ Lecithin and Polysorbate 80
- Control
- Pseudomonas aeruginosa
- Staphylococcus aureus
References
- Hall and Hartnett, 1964, Public Hith. Rep., 79:1021.
- Standard Methods for the Examination of Dairy Products. 17th Edition, 2004 Edited by H. Michael Wehr and Joseph H.Frank.
- MacFaddin J.F., 1985, Media for Isolation-Cultivation-Identification Maintenance of Medical Bacteria, Vol. I, Williams and Wilkins, Baltimore.
- Brummer, 1976, Appl. Environ. Microbiol., 32:80.
- Favero (Chairm), 1967, Biological Contamination Control Committee, a state of the art report., Am. Assoc. for contamination control.
- Lennettee, Spaulding and Truant (Eds.), 1974, Manual of Clinical Microbiology, 2nd ed., ASM, Washington, D.C.
| Product Name | Microbial Content Test HiVeg™ Agar |
|---|---|
| SKU | MV449 |
| Customized Product Available | No |
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