Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended use
Recommended for culturing wide variety of microorganisms, particularly obligate anaerobes from various specimens.
Composition
| Ingredients | g/L |
|---|---|
| HiVeg™ hydrolysate | 17.000 |
| Soya peptone | 3.000 |
| Dextrose (Glucose) | 6.000 |
| Sodium chloride | 2.500 |
| Sodium thioglycollate | 0.500 |
| L-Cystine | 0.250 |
| Sodium sulphite | 0.100 |
| Agar | 0.700 |
| Final pH (at 25°C) | 7.0±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 30.05 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the medium in an upright position. For maintenance of viability of cultures, add small amount of calcium carbonate into the containers before filling.
Principle And Interpretation
Thioglycollate Medium without Indicator is a semisolid medium originally formulated by Brewer (1) for the growth of aerobic and anaerobic microorganisms (2,3). Previously methylene blue was incorporated in the medium as an Eh indicator but has been omitted now to enable recognition of early growth and avoids any toxic effects of indicator. This medium supports a minimal inoculum with early visibility of growth. Obligate aerobes grow at the top of the medium, while anaerobes grow at the bottom of the medium.
This medium is nutritious and favours the growth of Clostridium butyricum, Campylobacter species, Bacteroides species and Pneumococci etc. from minimal inocula. Brucella species which fail to grow in the presence of indicator, can grow in this medium. The broth with addition of 10% v/v serum may be used for cultivation of Trichomonas vaginalis. It can also be used as transportation medium for which calcium carbonate is incorporated in the medium. Calcium carbonate neutralizes the acid produced during growth and avoid rapid growth and death of gram-negative cocci, Clostridium perfringens and other acid-sensitive bacteria. Diagnostic Thioglycollate HiVeg™ Medium is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. HiVeg™ hydrolysate, Soya peptone, dextrose, L-cystine provides nitrogenous and carbonaceous compounds, fermentable carbohydrate and trace elements. Sodium thioglycollate serves as a reducing agent. The small amount of agar helps in anaerobiosis. The reducing action provided by sodium thioglycollate and sodium sulphite binds molecular oxygen, thereby maintaining a low Eh (4). A small amount of agar is added to retard the absorption of oxygen by reducing convection currents in the medium.
Type of specimen
Samples
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Due to varying nutritional requirements, certain strain may show slow growth.
- Further biochemical and serological tests needs to be performed for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light amber coloured very slightly opalescent, viscous solution.
Reaction
Reaction of 3.0% w/v aqueous solution at 25°C. pH : 7.0±0.2
pH
6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| & Phocaeicola vulgatus ATCC 8482 | 50-100 | poor-fair |
| Clostridium sporogenes ATCC 11437 | 50-100 | good-luxuriant |
| Candida albicans ATCC 10231 (00054*) | 50-100 | good-luxuriant |
| $ Bacillus spizizenii ATCC 6633(00003*) | 50-100 | good-luxuriant |
| ^ Kocuria rhizophila ATCC 10240 | 50-100 | good-luxuriant |
| Neisseria meningitidis ATCC 13090 | 50-100 | good-luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | good-luxuriant |
Key: *Corresponding WDCM numbers (&) Formerly known as Bacteroides vulgatus ($) Formerly known as Bacillus subtilis subsp. spizizenii (^)Formerly known as Micrococcus luteus.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Diagnostic Thioglycollate HiVeg™ Medium (Thioglycollate HiVeg™ Medium w/o Indicator) |
|---|---|
| SKU | MV191 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Brewer J. H., 1940, J. Bacteriol., 39:10. 2.Vera H. D., 1944, J. Bacteriol., 47:59-70. 3.Hansen P. A., Price K. E. and Clements M. F., 1952, J. Bacteriol., 64:772. 4.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol.1 William andWilkins, Baltimore. 5.Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition. 6.Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1. |
| Customized Product Available | No |
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