Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg® peptone | 5.0 |
| Yeast extract | 3.0 |
| Agar | 15.0 |
Final pH (at 25°C) 7.5± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 23 grams in 990 ml distilled water. Add 10 ml of Tributyrin. Mix and heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Shake the flask and pour plate so as to maintain uniform turbidity.
Principle and Interpretation
Tributyrin HiVeg® Agar Base is specially developed from HiVeg® peptone to avoid BSE/ TSE risks associated with animal origin Peptic digest of animal tissue. This medium is the modification of Tributyrin Agar Base, which was originally formulated by Anderson (1) for the detection and enumeration of lipolytic microorganisms such as Staphylococci (2), Clostridia (3), marine Flavobacteria and Pseudomonas (4) and moulds in foodstuffs and other materials.
HiVeg® peptone and yeast extract provide necessary nutrients for growth of the microorganisms. Tributyrin added in the medium on hydrolysis by esterase enzyme produces butyric acid which is water soluble therefore clearance is seen around the colony which produce the enzyme. Lipases hydrolysing low molecular weight or short chain triglycerides are refered as esterases. This is an invitro method followed to isolate lipolytic organisms and is used for screening lipase/ esterase producers. The medium should be properly mixed so that tributyrin substrate is distributed uniformly in the prepared plate.
Quality Control
Appearance of powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and Clarity
Light yellow coloured, opalescent gel forms with oil droplets in petri plates.
Reaction
Reaction of 2.3% w/v aqueous solution containing 1% v/v Tributyrin is pH 7.5± 0.2 at 25°C
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
| MV157 | M157 |
| HiVeg® peptone | Peptic digest of animal tissue |
| Recommended for | : Detection of lipolytic microorganisms. |
| Reconstitution | : 23.0 g/l |
| Quantity on preparation (500g) | : 21.73 L |
| (100g) | : 4.34 L |
| pH (25°C) | : 7.5± 0.2 |
| Supplement | : Tributyrin (FD081) |
| Sterilization | : 121°C / 15 minutes |
| Storage | : Dry Medium - Below 30°C, Prepared Medium 2 - 8°C. |
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 24-48 hours, in an appropriate atmosphere after addition of Tributyrin (FD081).
Organisms (ATCC)
| Organisms (ATCC) | Inoculum (CFU) | Growth | Lipase activity |
|---|---|---|---|
| *Clostridium perfringens (12924) | 102-103 | luxuriant | - |
| *Clostridium sporogenes (11437) | 102-103 | luxuriant | + |
| Bacillus subtilis (6633) | 102-103 | luxuriant | + |
| Escherichia coli (25922) | 102-103 | luxuriant | - |
| Staphylococcus aureus (25923) | 102-103 | luxuriant | + |
Key :
- + = clear zone around colony
- - = absence of zone
- * = when incubated anaerobically
| Product Name | Tributyrin HiVeg® Agar Base w/o Tributyrin |
|---|---|
| SKU | MV157 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Alford J. A., and Steinle E. E., 1967, J. Appl. Bacteriol., 30: 488 2.American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed.,Washington D.C. 3.Anderson J. A., 1939, Ber, IIIrd Int. Mikrobiol. Kongress, 3 : 726 4.Collee J. G., Fraser A. G., Marmion B. P., Simmons A., (Eds.), Mackie and McCartney, Practical Medical Microbiology, 1996, 14th Edition, Churchill Livingstone 5.Frayer T. T., Lawrence R. C., Reiter B, 1967, J. Dairy Sci., 50: 477. 6.Hayes P. R., 1963, J. Gen. Microbiol., 30: 1 9.Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1. 10.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1,Williams and Wilkins, Baltimore 2.Vanderzant C. and Splittstoesser D. F., (Eds.), 1992, Compendium of Methods for the Microbiological Examination of Foods, 3rd Ed., APHA, Washington, D.C.1 3.Willis A. T., 1960, J. Path. Bacteriol., 80 (2): 37914.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc., Washington, D.C |
| Customized Product Available | No |
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Tributyrin Agar Base w/o Tributyrin
As low as
₹1,646.00
Tribut (10 ml per vial)
As low as
₹2,336.00