Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Intended Use
Brilliant Green HiVeg Broth 2% is recommended for the detection and confirmation of coliform bacteria in water, waste water, foods, milk and dairy products.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 25.0 |
| Lactose | 10.0 |
| Synthetic detergent No. II | 5.0 |
| Brilliant green | 0.0133 |
Final pH (at 25°C) 7.2 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 40 grams in 1000 ml distilled water. Heat if necessary to boiling to ensure complete solution. Mix well. Distribute in fermentation tubes containing inverted Durham's tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
Brilliant Green HiVeg Broth 2% is specially developed using HiVeg peptone to avoid BSE/TSE risks associated with animal origin peptone. This medium is the modification of Brilliant Green Bile Broth 2% which is used for presumptive identification and confirmation of coliform bacteria (1,2). Brilliant green and synthetic detergent No. Il present in the medium inhibits gram-positive bacteria. Production of gas from lactose fermentation detected by incorporating inverted Durham's tube, indicates a positive evidence of faecal coliforms since nonfaecal coliforms growing in this medium do not produce gas. During examination of water samples, growth from presumptive positive tubes showing gas in Lactose HiVeg Broth (MV026) or Lauryl Tryptose HiVeg Broth (MV080) is inoculated in Brilliant Green HiVeg Broth 2% wherein gas formation within 48 ± 2 hours confirms the presumptive test.
Product Specifications
Recommended for: Detection and confirmation of coliform bacteria in water, foods, milk and dairy products.
Reconstitution: 40.0 g/l
| Quantity on preparation (500g) | 12.5 L |
| (100g) | 2.5 L |
pH (25°C): 7.2 ± 0.2
Supplement: None
Sterilization: 121°C / 15 minutes.
Storage: Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Quality Control
Appearance of powder: Greenish yellow coloured, homogeneous, free flowing powder.
Colour and Clarity: Emerald green coloured, clear solution without any precipitate.
Reaction: Reaction of 4.0% w/v aqueous solution is pH 7.2 ± 0.2 at 25°C.
Cultural Response: Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 48 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Gas |
|---|---|---|---|
| Bacillus cereus (10876) | 102-103 | inhibited | - |
| Enterobacter aerogenes (13048) | 102-103 | luxuriant | + |
| Enterococcus faecalis (29212) | 102-103 | none-poor | - |
| Escherichia coli (25922) | 102-103 | luxuriant | + |
| Staphylococcus aureus (25923) | 102-103 | inhibited | - |
References
- McCrady and Langerin, 1932, J. Dairy Science, 15:321.
- McCrady, 1937, Am. J. Publ. Health, 27:1243.
| Product Name | Brilliant Green HiVeg™ Broth, 2% |
|---|---|
| SKU | MV121 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg), Lactose |
| Packaging type | HDPE |
| References | 1. Greenberg A. E., Eaton A. D. and Clesceri L. S., (Eds.), 1998, Standard Methods for the Examination of Water andWastewater, 20th ed., APHA, Washington, D.C. 2.Downes F. P. and Ito K. (Eds.) 2001, Compendium of Methods for the Microbiological Examination of Food. 4th Ed, APHA,Washington, D.C. 3.Richardson G., (Ed.), 1985, Standard Methods for the Examination of Dairy Products, 15th Ed, APHA, Washington, D.C. 4.McCrady and Langerin, 1932, J. Dairy Science, 15:321. 5.McCrady, 1937, Am. J. Publ. Health, 27:1243. 6.International Organization for Standardization (ISO), 1991, Draft ISO/DIS 4831. 7.Isenberg, H.D. Clinical Microbiology Procedures Handb0ook. 2nd Edition. 8.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1. |
| Customized Product Available | No |
Write Your Own Review
Brilliant Green Bile Broth, 2%
As low as
₹0.00
Upsell Products
Brilliant Green Agar Modified Plate
As low as
₹1,044.00