Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Fluid Casein Digest Soya Lecithin HiVeg Medium is recommended for sanitary examination of surfaces.
Composition
| Ingredients | Grams/Litre |
|---|---|
| Part A: HiVeg hydrolysate | 20.0 |
| Soya lecithin | 5.0 |
| Part B: Polysorbate 20 | 40.0 ml |
| Final pH (at 25°C) 7.3 ± 0.2 | |
Formula adjusted, standardized to suit performance parameters.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) | |
|---|---|---|
| Product Code | MV117 | M117 |
| Key Ingredient | HiVeg hydrolysate | Casein enzymic hydrolysate |
| Recommended for | Sanitary examination of surfaces. | |
| Reconstitution | 25.0 g/l (Part A)+ 40.0 ml/l (Part B) | |
| Quantity on preparation (500g) | 7.69 L (A+B) | |
| Quantity on preparation (100g) | 1.53 L (A+B) | |
| pH (25°C) | 7.3 ± 0.2 | |
| Supplement | None | |
| Sterilization | 121°C / 15 minutes. | |
| Storage | Dry Medium and Prepared Medium 2-8°C. | |
Directions
Suspend 25 grams of Part A in 960 ml distilled water. Heat if necessary to dissolve the medium completely. Add 40 ml of Part B. Mix well and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
This medium is prepared by using HiVeg hydrolysate is place of Casein enzymic hydrolysate which makes the medium free of BSE/TSE risks. Fluid Casein Digest Soya Lecithin HiVeg Medium is recommended for sanitary examination of surfaces. Weber and Black had described the importance of a highly nutritional medium containing the neutralizing agents for quaternary ammonium compounds (1,2).
The medium contains HiVeg hydrolysate which provides the necessary nutrients for the growth of the organisms. Soya lecithin neutralizes the quaternary ammonium compounds while polysorbate 20 neutralizes phenolic disinfectants, hexachlorophene and formalin (3).
Quality Control
Appearance of powder
Part A: Yellow coloured, may have greenish tinge, homogeneous, free flowing powder.
Part B: Colourless, clear, viscous liquid.
Colour and Clarity
Yellow coloured, clear solution without any precipitate.
Reaction
Reaction of the medium (2.5% w/v Part A + 4.0% v/v Part B) is pH 7.3 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth |
|---|---|---|
| *Candida albicans (10231) | 102-103 | good-luxuriant |
| Bacillus subtilis (6633) | 102-103 | good-luxuriant |
| Escherichia coli (25922) | 102-103 | good-luxuriant |
| Staphylococcus aureus (25923) | 102-103 | good-luxuriant |
Key: * = Incubate at 25-30°C for 24-48 hours.
References
- Weber and Black, 1948, Soap and Sanitary Chemicals, 24:134.
- Weber and Black, 1948, Am. J. Public Health, 38:1405.
- Favero (chem.), 1967, Microbiological Sampling of Surfaces, Biological Contamination Control Committee, American Asso. for Contamination Control.
| Product Name | Fluid Casein Digest Soya Lecithin HiVeg™ Medium (Twin Pack) |
|---|---|
| SKU | MV117 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. The United States Pharmacopeia, 2009, The United States Pharmacopeial Convention. Rockville, MD. 2.Indian pharmacopoeia, 1997, Govt. of India, Ministry of Health and Family Welfare, Vol. II, Controller of Publications,New Delhi. 3.Weber and Black, 1948, Soap and Sanitary Chemicals, 24:134. 4.Weber and Black, 1948, Am. J. Public Health, 38:1405. 5.National Aeronautics and Space Administration, 1966, Standard Procedures for the Microbiological Examination of SpaceHardware. 6.Favero (chm.), 1967, Microbiological Sampling of Surfaces, Biological Contamination Control Committee, American Asso.for Contamination Control. |
| Customized Product Available | No |
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