Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Gassner Lactose HiVeg Agar is used for detection and isolation of pathogenic Enterobacteriaceae from food stuffs and other materials.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) | ||
|---|---|---|---|
| MV1022 | HiVeg peptone No. 1 | M1022 | Meat peptone |
Recommended for : Detection and isolation of pathogenic Enterobacteriaceae from food stuffs and other materials.
Reconstitution : 76.87 g/l
Quantity on preparation (500g): 6.5 L
pH (25°C) : 7.2 ± 0.2
Supplement : None
Sterilization : 121°C / 15 minutes.
Storage : Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone No.1 | 7.00 |
| Sodium chloride | 5.00 |
| Lactose | 50.00 |
| Metachrome yellow | 1.25 |
| Water blue | 0.625 |
| Agar | 13.00 |
| Final pH (at 25°C) | 7.2 ± 0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 76.87 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
This medium is prepared by replacing animal based Meat peptone by HiVeg peptone No. 1, therefore the medium is free of TSE/BSE risk. Gassner Lactose HiVeg Agar is the modification of Gassner Lactose Agar originally developed by Gassner for the detection and isolation of pathogenic Enterobacteriaceae from food and other materials (1). This medium is also known as Water-blue Metachrome-yellow Lactose Agar. Metachrome-yellow primarily inhibits gram-positive microorganisms present in the food materials. Water blue indicator turns blue in acidic range and colourless in alkaline range. Lactose fermenters produce acid and therefore change the colour of the medium. Original colour of the prepared medium is green, but in the acidic pH range it becomes blue-green to blue while at alkaline range the yellow colour of metachrome yellow becomes increasingly apparent. Medium ingredients like HiVeg peptone No. 1 and sodium chloride provides nutrients and maintains osmotic balance respectively. Metachrome yellow and water blue are the pH indicator dyes.
Quality Control
Appearance of Powder
Greenish yellow, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.3% Agar gel.
Colour and Clarity
Dark green coloured, clear to slightly opalescent gel forms in petri plates.
Reaction
Reaction of 7.69% w/v aqueous solution is pH 7.2 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organisms | Inoculum | Growth | Recovery | Colour of Colony* | Colour change of medium |
|---|---|---|---|---|---|
| Enterococcus faecalis (29212) | 102-103 | inhibited | 0% | — | — |
| Escherichia coli (25922) | 102-103 | good -luxuriant | >70% | dark green | blue |
| Klebsiella pneumoniae (13883) | 102-103 | good -luxuriant | >70% | mucoid green | blue |
| Proteus mirabilis (25933) | 102-103 | good -luxuriant | >70% | yellowish green | yellow |
| Salmonella serotype Typhi (6539) | 102-103 | good -luxuriant | >70% | yellow | yellow |
| Salmonella serotype Typhimurium (14028) | 102-103 | good -luxuriant | >70% | yellow | yellow |
| Salmonella serotype Enteritidis (13076) | 102-103 | good -luxuriant | >70% | yellow | yellow |
| Shigella flexneri (12022) | 102-103 | good -luxuriant | >70% | yellow | yellow |
| Staphylococcus aureus (25923) | 102-103 | inhibited | 0% | — | — |
Note: *Lactose nonfermenters show light green to yellow green surrounded by a yellowish zone. Lactose fermenter like E.coli, coliform bacteria and others show deep blue colony surrounded by a blue zone. However due to background colour of agar medium, slight variation in observed colour is visualized as interpreted in column above.
References
- Gassner G., 1918, Centralbl. F. Bakt. I. Orig., 80:219.
| Product Name | Gassner Lactose HiVeg™ Agar |
|---|---|
| SKU | MV1022 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.Gassner G., 1918, Centralbl. F. Bakt. I. Orig., 80:219-22 |
| Customized Product Available | No |
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