Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for dextrose fermentation studies of microorganisms.
Composition
| Ingredients | g/L |
|---|---|
| HiVeg® peptone No. 3 | 10.000 |
| HiVeg® extract | 1.000 |
| Sodium chloride | 5.000 |
| Dextrose (Glucose) | 5.000 |
| Phenol red | 0.018 |
| Final pH (at 25°C) | 7.4±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 21.02 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes containing inverted Durham's tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C.
Principle And Interpretation
Phenol Red Broth Medium is formulated as per Vera (1) and is recommended to determine the fermentation reaction of carbohydrates for the differentiation of microorganisms (2,3,4). Phenol Red Broth Medium with various added carbohydrates serves as a differential medium by aiding in differentiation of various species and genera by their ability to ferment the specific carbohydrate, with the production of acid or acid and gas (5). Phenol Red Dextrose HiVeg® Broth Base is same as Phenol Red Dextrose Broth Base except that the animal based peptones are completely replaced with vegetable peptones to avoid the BSE/TSE risks associated with animal peptones.
HiVeg® peptone No. 3 and HiVeg® extract serve as sources for carbon and nitrogen. Sodium chloride is the osmotic stabilizer. Phenol red is the pH indicator, which turns yellow at acidic pH. Gas formation is seen in Durham's tubes. All of the Enterobacteriaceae grow well in this medium. In addition to producing a pH colour shift, the production of mixed acids, notably butyric acids, often results in a pungent, foul odour from the culture medium (6).
Type of specimen
Isolated Microorganism
Specimen Collection and Handling
For isolated microorganism samples follow appropriate techniques for handling specimens as per established guidelines. After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- This media is not intended for primary isolation of specimens.
- Overincubation is not recommended
- Since the medium is pH dependent, adjustment of pH is important for correct results.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Light yellow to pink coloured homogeneous free flowing powder
Colour and Clarity of prepared medium
Red coloured clear solution without any precipitate
Reaction
Reaction of 2.1% w/v aqueous solution at 25°C. pH : 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours (longer if necessary).
| Organism | Growth | Acid | Gas |
|---|---|---|---|
| Citrobacter freundii ATCC 8090 | luxuriant | Positive reaction, yellow colour | Positive reaction |
| Escherichia coli ATCC 25922 (00013*) | luxuriant | Positive reaction, yellow colour | Positive reaction |
| # Klebsiella aerogenes ATCC 13048 (00175*) | luxuriant | Positive reaction, yellow colour | Positive reaction |
| Klebsiella pneumoniae ATCC 13883 (00097*) | luxuriant | Positive reaction, yellow colour | Positive reaction |
| $ Proteus hauseri ATCC 13315 | luxuriant | Positive reaction, yellow colour | Positive reaction |
| Salmonella Typhi ATCC 6539 | luxuriant | Positive reaction, yellow colour | Positive reaction |
| Salmonella Typhimurium ATCC 14028 (00031*) | luxuriant | Positive reaction, yellow colour | Positive reaction |
| Serratia marcescens ATCC 8100 | luxuriant | Positive reaction, yellow colour | Positive reaction |
| Shigella flexneri ATCC 12022 (00126*) | luxuriant | Positive reaction, yellow colour | Negative reaction |
Key: (*) Corresponding WDCM numbers.
(#) Formerly known as Enterobacter aerogenes
$ Formerly known as Proteus vulgaris
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | Phenol Red Dextrose HiVeg™ Broth |
|---|---|
| SKU | MV056 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Koneman E. W., Allen S. D., Janda W.M., Schreckenberger P.C., Winn W.C. Jr., 1992, Colour Atlas and Textbook ofDiagnostic Microbiology, 4th Ed., J. B. Lippinccott Company 2.Vera H. D., 1950, Am. J. Public Health, 40, 1267 3.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification -Maintenanceof Medical Bacteria, Vol. I, Williamsand Wilkins, Baltimore. 4.Finegold S. M. and Baron E. J., 1986, Bailey and Scotts Diagnostic Microbiology, 7th Ed., The C.V. Mosby Co., St. Louis. 5.Ewing W. H., 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th ed.,Elsevier Science Publishing Co.,Inc., New York. 6.MacFaddin J. F., 2000, Biochemical tests for Identification of Medical Bacteria, 3rd edi., Lippincott, Williams and Wilkins,Baltimore. |
| Customized Product Available | No |
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