Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Tomato Juice HiVeg Agar / Special is used for the cultivation and enumeration of Lactobacilli.
Composition
| Ingredients | MV048 Grams/Litre | MV879 Grams/Litre |
|---|---|---|
| Tomato juice (400 ml) | 20.00 | 20.00 |
| HiVeg hydrolysate | 10.00 | — |
| HiVeg peptone | — | 10.00 |
| HiVeg hydrolysate No. 3 | 10.00 | 10.00 |
| Agar | 11.00 | 20.00 |
| Final pH (at 25°C) | 6.1 ± 0.2 | 5.0 ± 0.2 |
Formula adjusted, standardized to suit performance parameters.
Product Profile
Vegetable based (Code MV)
MV048/MV879
- HiVeg peptone
- HiVeg hydrolysate
- HiVeg hydrolysate No. 3
Animal based (Code M)
M048/M879
- Peptic digest of animal tissue
- Casein enzymic hydrolysate
- Peptonized milk
Recommended for
Cultivation and enumeration of Lactobacilli.
Reconstitution
- (MV048): 51.0 g/l
- (MV879): 60.0 g/l
Quantity on preparation (500g)
- (MV048): 9.80 L
- (MV879): 8.33 L
pH (25°C)
- (MV048): 6.1 ± 0.2
- (MV879): 5.0 ± 0.2
Supplement
None
Sterilization
121°C / 15 minutes.
Storage
Dry Medium and Prepared Medium 2 - 8°C.
Directions
Suspend 51 grams of MV048 or 60 grams of MV879 in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
These media are prepared by using vegetable peptones in place of animal based peptones which makes the media free of BSE/TSE risks. Mickle and Breed (1) first described the use of tomato juice in the culture media for Lactobacilli. These media are the modifications of media developed by Kulp (2) who observed the improved growth of Lactobacillus acidophilus in the media consisting of tomato juice. Kulp and White (3) observed that high counts of Lactobacilli were seen on using Tomato Juice. Tomato Juice Agar media are used for the cultivation and enumeration of Lactobacilli. Tomato Juice HiVeg Agar/Special which are modifications of the conventional media serve the same purpose.
The number of Lactobacilli in saliva is an index of predisposition to dental caries as described by Jay (4). Tomato Juice HiVeg Agar, Special like the conventional medium is recommended for the direct plate count of the Lactobacilli in saliva and for other acidophilic organisms. The low pH of medium inhibits many commensal bacteria and encourages the growth of Lactobacilli.
Tomato juice provides an acidic environment, besides being a source of carbon, proteins and nutrients. HiVeg hydrolysate No.3 provides lactose, as an energy source. HiVeg peptone and HiVeg hydrolysate provides nitrogenous, carbonaceous compounds, trace elements and other essential growth nutrients.
Gelling
Firm, comparable with 1.1% Agar gel of MV048 or 2.0% Agar gel of MV879.
Colour and Clarity
Medium amber coloured, slightly opalescent gel forms in petri plates.
Reaction
Reaction of 5.1% w/v aqueous solution of MV048 is pH 6.1 ± 0.2 at 25°C. Reaction of 6.0% w/v aqueous solution of MV879 is pH 5.0 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 40-48 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth on MV048 | Growth on MV879 |
|---|---|---|---|
| Lactobacillus acidophilus (4356) | 102-103 | luxuriant | luxuriant |
| Lactobacillus casei (9595) | 102-103 | luxuriant | luxuriant |
| Lactobacillus leichmannii (4797) | 102-103 | luxuriant | luxuriant |
| Staphylococcus aureus (25923) | 102-103 | luxuriant | inhibited |
Quality Control
Appearance of powder
Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
| Product Name | Tomato Juice HiVeg™ Agar |
|---|---|
| SKU | MV048 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.Mickle F. L. and Breed R. S., 1925, Technical Bulletin 110, N.Y. State Agriculture Exp. Station, Geneva, N.Y. 2.Kulp W. L., 1927, Science 66:512. 3.Kulp W. L. and White V., 1932, Science 76:17. 4.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams& Wilkins, Baltimore, Md. |
| Customized Product Available | No |
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