Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Intended Use
Hoyle HiVeg Medium Base is a highly selective medium used for the isolation and differentiation of Corynebacterium diphtheriae.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 10.00 |
| HiVeg extract | 10.00 |
| Sodium chloride | 5.00 |
| Agar | 15.00 |
| Final pH (at 25°C) | 7.8 ± 0.2 |
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 40 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 55°C and aseptically add 50 ml of laked blood and 10 ml of 3.5% Potassium Tellurite solution (FD047). Mix well and pour into sterile plates.
Principle and Interpretation
Hoyle HiVeg Medium Base is prepared by using HiVeg peptone and HiVeg extract in place of animal based peptones, making the medium free of BSE/TSE risks. This medium is the modification of the medium modified by Hoyle (1) originally formulated by Neill for the isolation and differentiation of Corynebacterium diphtheriae. It, like the conventional medium is less inhibitory to some mitis types as compared to Neill's medium but supports very rapid growth of all types of Corynebacterium diphtheriae so that diagnosis is possible after 18 hours incubation. HiVeg peptone and HiVeg extract supply essential growth nutrients. Potassium tellurite is a selective agent which inhibits most of the normal flora of the upper respiratory tract except Corynebacterium. Hoyle HiVeg Medium Base is a highly selective medium and should be used in conjunction with non-selective media such as Loeffler Serum HiVeg Medium (MV537) and Blood Agar Base w/low pH, HiVeg (MV089) with 10% horse blood (2). Corynebacterium diphtheriae are usually present in small numbers permitting well isolated colonies. So, inoculation is done by directly rubbing the swab over the entire surface of the medium. Incubation should be carried out till 72 hours if the results are negative. To study the morphology, Gentian violet staining is done. To demonstrate the characteristic morphology and staining reactions of Corynebacterium diphtheriae by Neisser's or Alberts method, it is advisable to use colonies from Loeffler medium. The toxigenicity of Corynebacterium diphtheriae strains can be determined by Elek's (3) method.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV015 HiVeg peptone HiVeg extract |
M015 Peptic digest of animal tissue Beef extract |
Recommended for
Isolation and differentiation of Corynebacterium diphtheriae.
Reconstitution
40.0 g/l
Quantity on preparation
(500g): 12.5 L
(100g): 2.5 L
pH (25°C)
7.8 ± 0.2
Supplement
Laked blood, 3.5% Potassium Tellurite Solution (FD047)
Sterilization
121°C / 15 minutes.
Storage
Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Quality Control
Appearance of Powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and Clarity
Basal medium yields amber coloured gel, addition of laked blood and tellurite gives brownish red coloured, opalescent gel forms in petri plates.
Reaction
Reaction of 4.0% w/v aqueous solution is pH 7.8 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours, with added 50 ml of laked blood and 10 ml of 3.5% Potassium Tellurite Solution (FD047).
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery | Colony characteristics |
|---|---|---|---|---|
| Corynebacterium diphtheriae type mitis | 102-103 | good-luxuriant | >50% | Grey colonies with shinning surface. |
| Corynebacterium diphtheriae type intermedius (14779) | 102-103 | good-luxuriant | >50% | Grey colonies with darker centres. |
| Enterococcus faecalis (29212) | 102-103 | good-luxuriant | >50% | Black minute colonies |
| Escherichia coli (25922) | 102-103 | inhibited | 0% | — |
References
- Hoyle I., 1941, Lancet., 1:175.
- MacFaddin J.F., 1985 (ed), Media for Isolation-Cultivation-Identification Maintenance of Medical Bacteria, Vol I, William and Wilkins, Baltimore.
- Elek S.D., 1948, Brit. Med. J., 1:493.
| Product Name | Hoyle HiVeg™ Medium Base |
|---|---|
| SKU | MV015 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H.,8th Ed., American Society for Microbiology, Washington, D.C. (Ed.), 2003, Manual of Clinical Microbiology,2.Hoyle I., 1941, Lancet., 1:175. 3.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williamsand Wilkins, Baltimore. 4.Elek S. D., 1948, Brit. Med. A1:493. 5.Isenberg, H.D. Clinical Microbiology Procedures Handb0ook. 2nd Edition. 6.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1. |
| Customized Product Available | No |
Write Your Own Review
Hoyle Medium Base
As low as
₹1,522.00
PTe 3.5% Selective Supplemen (1 ml per vial)
As low as
₹1,009.00
Upsell Products
Hoyles Media Plate with supplements
As low as
₹4,490.00