Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for isolation, cultivation and differentiation of Corynebacterium diphtheriae.
Composition
| Ingredients | g/L |
|---|---|
| Acicase™ # | 20.000 |
| M-Protein powder $ | 5.000 |
| Potassium dihydrogen phosphate | 0.300 |
| Magnesium sulphate heptahydrate | 0.100 |
| L-Tryptophan | 0.050 |
| Agar | 20.000 |
| Final pH (at 25°C) | 7.4±0.1 |
| MTS Supplement (25 ml per vial)(FD100) | Concentration |
| Potassium tellurite | 100mg |
| Calcium D pantothenate | 0.050mg |
| Horse serum | 12.500ml |
| Sodium lactate solution | 10.000ml |
| Ethanol | 2.500ml |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Casein acid hydrolysate
$ Equivalent to Casein powder
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Corynebacterium diphtheriae is gram-positive, facultative anaerobic, non-motile bacteria. It is the etiological agent for diphtheria. Many species of Corynebacteria can be isolated from various places such as soil, water, blood, and human skin. Pathogenic strains of Corynebacteria can infect plants, animals, or humans. Though humans are now the only known reservoir for the disease. The bacterium is generally found in temperate zones but may also be found in other parts of the world. Various tellurite media such as Mcleods (1), Hoyles (2), or CTBA (3) have been used for isolation and differentiation of C.diphtheriae. Mueller Tellurite Agar has been recommended (4) for isolation, cultivation and differentiation of C. diphtheriae. Potassium tellurite in the medium inhibits the growth of most of the normal flora of the upper respiratory tract allowing C.diphtheriae and other saprophytic Corynebacteria to grow. The serum used in medium enhances granule formulation. Acicase™ and L-tryptophan provide nitrogenous compounds. Magnesium sulphate supplies essential ions required by the organisms. C.diphtheriae forms grayish black colonies surrounded by dark brown halo due to production.
Type of specimen
Clinical samples - Throat swab; Water samples
Specimen Collection and Handling
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (5). For clinical samples follow appropriate techniques for handling specimens as per established guidelines (6,7). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- 1. Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- 2. Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Mueller Tellurite Agar in 90 mm disposable plates with smooth surface and absence of black particles/cracks/bubbles
Colour
Colourless to light yellow coloured medium.
Quantity of Medium
25ml of medium in 90 mm disposable plates.
pH
7.30-7.50
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Corynebacterium diphtheriae ATCC 11913 | 50-100 | luxuriant | >=50% |
| Corynebacterium xerosis ATCC 7094 | 50-100 | luxuriant | >=50% |
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).
| Product Name | Mueller Tellurite Agar Plate |
|---|---|
| SKU | MP1202 |
| Customized Product Available | No |
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