Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended use
For isolation of Neisseria gonorrhoeae from chronic and acute gonococcal infections.
Composition
| Ingredients | g/L |
|---|---|
| Proteose peptone | 20.000 |
| Dextrose | 0.500 |
| Sodium chloride | 5.000 |
| Disodium phosphate | 5.000 |
| Agar | 15.000 |
| After sterilization | |
| Sterile Lysed blood (at 80°C) | 50.000 |
| Vitamino Growth Supplement (FD025) | 2 vials |
| Final pH (at 25°C) | 7.3±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Neisseria gonorrhoeae is a gram-negative bacteria and the causative agent of gonorrhea, however it is also occasionally found in the throat. The cultivation medium for gonococci should ideally be a rich nutrients base with blood, either partially lysed or completely lysed. The diagnosis and control of gonorrhea have been greatly facilitated by improved laboratory methods for detecting, isolating and studying N. gonorrhoeae.
Chocolate Agar Base, with the addition of supplements, gives excellent growth of the gonococcus without overgrowth by contaminating organisms. G.C. Agar (M434) can also be used in place of Chocolate Agar Base, which gives slightly better results than Chocolate Agar (1). The diagnosis and control of gonorrhea have been greatly facilitated by improved laboratory methods for detecting, isolating and studying N. gonorrhoea.
Interest in the cultural procedure for the diagnosis of gonococcal infection was stimulated by Mcleod and co-workers (2), Carpenter and Morton (3) and others. who clearly demonstrated the superiority of this method over the microscopic technique. Chocolate Agar Base with addition of supplement not only supports the growth of the gonococcus in pure culture but also permits its development from the mixed flora encountered in chronic gonococcal infections. Carpenter (4) reported that this medium and Haemoglobin (FD022) is useful for cultural detection of the gonococcus.
Type of specimen
Clinical samples - vaginal swabs
Specimen Collection and Handling:
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (5,6).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
In Vitro diagnostic Use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Some strains may show poor growth due to varying nutritional requirements.
- Further biochemical tests must be carried out for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Chocolate Agar in 90mm disposable plate with smooth surface and absence of black particles/bubbles/cracks.
Colour
Chocolate brown coloured medium
Quantity of medium
25ml of medium in 90mm plate
pH
7.10-7.50
Sterility Check
Passes release criteria
Cultural response
Cultural characteristics observed after an incubation at 35-37°C for 24 - 48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Neisseria gonorrhoeae ATCC 19424 | 50-100 | luxuriant | >=70% |
| Neisseria meningitidis ATCC 13090 | 50-100 | luxuriant | >=70% |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | luxuriant | >=70% |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant | >=70% |
| Haemophilus influenzae ATCC 19418 | 50-100 | luxuriant | >=70% |
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
Reference
- Collee J. G., Fraser A. G., Marmion B. P., Simmons A., (Eds.), 1996, Mackie and McCartney, Practical Medical Microbiology, 14th Ed., Chruchill Livingstone.
- McLeod J. W., Cootes J. C., Happold F. C., Priestely D. P., Wheatley B., 1934, J. Path. Bacteriol., 39:221.
- Carpenter C. M., and Marton H. E., 1947, Proc. N. Y. State Assoc. Public Health Loss., 27:58.
- Carpenter C. M., Bucca M. A., Buck T. C., Casman E. P., VhristensenC. W., Crowe E., Drew R., Hill J., Lankford L. E., Morton H. E., PeizerL. R., Shaw C. J., and Thayer J. D., 1949, Am. J. Syphil. Gonorrh. Vene ral Diseases, 33:164.
- Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
- Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.
| Product Name | Chocolate Agar Plate |
|---|---|
| SKU | MP103 |
| Customized Product Available | No |
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