Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Intended Use
Recommended for confirmation of the presumptive test for members of the coliform group from clinical and non-clinical samples.
Composition**
| Ingredients | g/L |
|---|---|
| Peptone | 10.000 |
| Lactose | 10.000 |
| Dipotassium phosphate | 3.500 |
| Sodium sulphite | 2.500 |
| Basic fuchsin | 0.500 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.5±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Endo Agar was developed by Endo to differentiate gram-negative bacteria on the basis of lactose fermentation, while inhibiting gram-positive bacteria (1). Inhibition of the later was achieved without the use of bile salts as was traditionally used. Endo was successful in inhibiting gram-positive bacteria on his medium by the incorporation of sodium sulphite and basic fuchsin. The resulting Endo Agar, also known as Fuchsin Sulphite and Infusion Agar, was used to isolate the typhoid bacilli. Many modifications of this media have been done over the years. Endo Agar is recommended by APHA as an important medium in the microbiological examination of water and wastewater, dairy products and foods (2,3,4). Endo Agar is used to confirm the detection and enumeration of coliform bacteria following presumptive test of drinking water. It is also used for the detection and isolation of coliforms and faecal coliform from milk, dairy products and food. The medium contains peptone which provide nitrogen, carbon, vitamins and minerals required for bacterial growth. Sodium sulphite and basic fuchsin make this medium selective by suppressing gram-positive organisms. Coliforms produce pink colonies on fermentation of lactose while lactose non-fermenters produce colourless colonies on the medium. With Escherichia coli, this reaction is very pronounced as the fuchsin crystallizes, exhibiting a permanent greenish metallic luster (fuchsin luster) to the colonies. Medium should be stored away from light to avoid photo-oxidation
Type of specimen
Clinical samples - faeces; Food and dairy samples; Water samples
Specimen Collection and Handling:
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards(2). For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (3,4). For clinical samples follow appropriate techniques for handling specimens as per established guidelines (5,6). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
In Vitro diagnostic Use. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Besides Enterobacteriaceae, other gram negative bacteria and yeasts may also grow.
- Avoid exposure of the medium to light, as it may lead to photo oxidation and decrease productivity of the medium.
- Overheating of the medium must be avoided, as it may destroy the productivity of the medium.
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Endo Agar in 90 mm disposable plates with smooth surface and absence of black particles/cracks/bubbles.
Colour of medium
Orangish pink coloured medium
Quantity of medium
25 ml of medium in 90 mm disposable plates.
pH
7.30-7.70
Sterility check
Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) |
Growth | Recovery | Colour of Colony |
|---|---|---|---|---|
| ** Bacillus spizizenii ATCC 6633 (00003*) |
>=10³ | inhibited | 0% | |
| $Klebsiella aerogenes ATCC 13048 (00175*) |
50-100 | good-luxuriant | >=50% | pink |
| Enterococcus faecalis ATCC 29212 (00087*) |
50-100 | none-poor | <=10% | pink, small |
| Escherichia coli ATCC 25922 (00013*) |
50-100 | good-luxuriant | >=50% | pink to rose red with metallic sheen |
| Klebsiella pneumoniae ATCC 13883 (00097*) |
50-100 | good-luxuriant | >=50% | pink, mucoid |
| ## Proteus hauseri ATCC 13315 |
50-100 | good-luxuriant | >=50% | colourless to pale pink |
| Pseudomonas aeruginosa ATCC 27853 (00025*) |
50-100 | good-luxuriant | >=50% | colourless, irregular |
| Salmonella Typhi ATCC 6539 |
50-100 | good-luxuriant | >=50% | colourless to pale pink |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) |
>=10³ | inhibited | 0% | |
| Enterobacter cloacae ATCC 13047 (00083*) |
50-100 | good | 40-50% | pink |
| Salmonella Typhimurium ATCC 14028 (00031*) |
50-100 | good-luxuriant | >=50% | colourless |
| Salmonella Enteritidis ATCC 13076 (00030*) |
50-100 | good-luxuriant | >=50% | colourless |
| Shigella flexneri ATCC12022 (00126*) |
50-100 | good-luxuriant | >=50% | colourless |
Key: *Corresponding WDCM numbers.
## Formerly known as Proteus vulgaris
**-Formerly known as Bacillus subtilis subsp. spizizenii. $- Formerly known as Enterobacter aerogenes
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Endo Agar Plate |
|---|---|
| SKU | MP029 |
| Customized Product Available | No |
Write Your Own Review