5X Bradford Reagent

5X Bradford Reagent for Protein Estimation is formulated for rapid and accurate quantitative estimation of protein samples by a method developed by Marion M. Bradford . This reagent is conveniently supplied as a 5X concentrate solution which can be used as it is or by diluting to 1X form. The Bradford protein assay is based upon the formation of complexes between Coomassie Brilliant Blue G-250 and the protein samples in solution. When the protein sample binds to the dye the color of the solution turns blue from brown and there is a shift in the absorption maximum of the dye from 465 nm to 595 nm. This dye binding procedure is completed within 5 minutes and the blue colored complex formed is stable for one hour.