The HiPurA® 96 Plasmid DNA Purification Kit provides a simple and rapid method for purifying plasmid or cosmid DNA from small volume of bacterial cultures (2.0 ml). This kit is not recommended for the purification of large recombinant constructs such as BACs and PACs. The kit uses the principle of silica binding in a 96‐well format that eliminates the need for expensive resins, alcohol precipitation and hazardous organic compounds such as phenol and chloroform. This procedure is based on a modified alkaline lysis procedure specialized for high‐throughput DNA applications.
The HiPurA® 96 Plasmid DNA Purification Kit provides a simple and rapid method for purifying plasmid or cosmid DNA from small volume of bacterial cultures (2.0 ml). The kit uses the principle of silica binding in a 96-well format facilitating the binding, washing and elution steps thus enabling multiple samples to be processed simultaneously. This procedure is based on a modified alkaline lysis procedure specialized for high-throughput DNA applications. Concentration of DNA can be estimated by agarose gel electrophoresis. Typical DNA yield is upto 20 µg/well from 1-2ml cell culture/well.