Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Complete Supplement Mixture w/o HIS-LEU
Complete supplement for amino acids to prepare appropiate media for selection of HIS3 and LEU2 auxotrophic strains and transformants
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Saccharomyces cerevisiae and Schizosaccharomyces Pombe#902790
Intended Use
Complete Supplement Mixture w/o HIS-LEU is used as a dropout supplement for all strains of Saccharomyces cerevisiae.
Composition**
| Ingredients | Milligrams/Litre |
|---|---|
| Adenine | 10.00 |
| L-Arginine HCl | 50.00 |
| L-Aspartic acid | 80.00 |
| L-Isoleucine | 50.00 |
| L-Lysine HCl | 50.00 |
| L-Methionine | 20.00 |
| L-Phenylalanine | 50.00 |
| L-Threonine | 100.00 |
| L-Tryptophan | 50.00 |
| L-Tyrosine | 50.00 |
| Uracil | 20.00 |
| L-Valine | 140.00 |
** Formula adjusted, standardized to suit performance parameters
Directions
Suspend 670 mg in 1000 ml distilled water. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Mix well and dispense as desired.
Principle and Interpretation
Complete Supplement Mixture w/o HIS-LEU is used as a dropout supplement for all strains of Saccharomyces cerevisiae. Complete Supplement Mixture (CSM) is composed of all the amino acids required for the vigorous growth of Saccharomyces cerevisiae. It also contains uracil. CSM w/o HIS-LEU does not contain histidine and leucine. This makes it a double dropout growth medium for yeast cells. It can be mixed with yeast nitrogen base (YNB), ammonium sulphate and an appropriate carbon source (glucose/galactose/raffinose). A histidine and leucine auxotrophic yeast mutant strain cannot grow on this media but a wild-type or a histidine and leucine prototrophic yeast strain can grow.
Quality Control
Appearance of Powder: White to light yellow coloured, homogeneous, free flowing powder.
Colour and Clarity: Colourless to pale yellow coloured, clear solution without any precipitate.
Cultural Response: Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.
Organisms (ATCC)
| Organisms (ATCC) | Growth |
|---|---|
| Saccharomyces cerevisiae ATCC 9763 | good-luxuriant |
Storage and Shelf-life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Complete Supplement Mixture w/o HIS-LEU |
|---|---|
| SKU | G120 |
| Customized Product Available | No |
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