Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Tetrathionate Broth Medium
An enrichment broth for isolation of Salmonellae from specimens suspected to be contaminated with Salmonellae in accordance with Indian Pharmacopoeia 1996.
Your enquiry has been submitted
Please login to Post an Enquiry.
Intended Use
Recommended an enrichment broth for isolation of Salmonellae from specimens suspected to be contaminated with Salmonellae in accordance with IP.
Composition**
| Ingredients | Gms/Litre |
|---|---|
| HM peptone B # | 0.900 |
| Peptone | 4.500 |
| Yeast extract | 1.800 |
| Sodium chloride | 4.500 |
| Calcium carbonate | 25.000 |
| Sodium thiosulphate | 40.700 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract
Directions
Suspend 77.40 grams in 980 ml purified/ distilled water. Heat just to boiling. Cool below 45-50°C and aseptically add 20 ml iodine solution (6 grams of iodine and 5 grams of potassium iodide in 20 ml distilled water). Mix well and dispense in 10 ml quantities in sterile tubes. This complete medium should be used on the day of preparation. Do not heat after the addition of iodine solution. Use the medium immediately after addition of iodine.
Note: Due to presence of calcium carbonate, the prepared medium forms opalescent solution with a white precipitate.
Note: Directions specified are as per the concurrent edition of pharmacopoeia in force. Specified expiry period corresponds to this. User must ensure its compatibility with the latest edition.
Principle And Interpretation
Tetrathionate Broth Medium was originally described by Mueller (1) and found that the medium selectively inhibit coliforms and permit unrestricted growth of enteric pathogens. The medium is now formulated according to Indian Pharmacopoeia (2). Compendium of Microbiological Examination of Foods(3) and Standard Methods for the Examination of Water and Wastewater (4) specify this medium as enrichment medium for Salmonella species. Salmonella is the common causative agent of mild gastroenteritis to typhoid. It is common contaminant in food and other biological products. This medium supports the rejuvenation of Salmonella cells injured by food processing which are incapable of forming colonies on plate, but on injection can cause infection.
The selectivity depends on the ability of thiosulphate and tetrathionate (formed by addition of Iodine and Potassium iodide) in combination to suppress commensal coliform organisms (5,6). The microorganism harboring tetrathionate reductase flourish in this broth. Sodium thiosulphates are inactivators of halogens and can minimize its toxicity in the testing sample, if any during microbial limit tests. Yeast extract, HM peptone B and peptone provides essential nutrients, growth factors and vitamins in this medium. Calcium carbonate neutralizes the acidic tetrathionate decomposition products. Sodium chloride maintains osmotic balance For further confirmation, streak the enriched cultures after incubation, on the plates of Brilliant Green Agar (MM016), MacConkey Agar (MM081), Bismuth Sulphite Agar (MM027).
Type of specimen
Pharmaceutical samples
Specimen Collection and Handling
For pharmaceutical products, follow appropriate techniques for sample processing in case of viscous materials as mentioned under sterility (2). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- For further confirmation, streak the enriched cultures after incubation, on the plates of Brilliant Green Agar (MM016), MacConkey Agar (MM081), Bismuth Sulphite Agar (MM027).
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium: Complete medium with added brilliant green and iodine solution - Light green opalescent with white precipitate, on standing the precipitate settles down.
Growth Promotion Test: As per Indian Pharmacopoeia.
Cultural Response: Cultural characteristics observed with added brilliant green and iodine solution when sub cultured on Xylose Lysine Deoxycholate Agar (MM031) after enrichment in Tetrathionate medium, after an incubation at 35-37°C for 18-72 hours.
| Organism | Inoculum (CFU) | Growth | Observed Lot Recovery value (CFU) | Colour of Colony | Incubation temperature |
|---|---|---|---|---|---|
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant | 25-100 | red with black centres | 18-72 hrs |
| Salmonella Abony NCTC 6017 (00029*) | 50-100 | good-luxuriant | 25-100 | red with black centres | 18-72 hrs |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | Tetrathionate Broth Medium |
|---|---|
| SKU | MM032 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Mueller, 1923, Compt. Rend. Sco. Biol., 89:434. 2.The Indian Pharmacopoeia (1996), Vol. II. 3.Downes F P and Ito K(Eds.), 2001, Compendium of Methods For The Microbiological Examination of Foods, 4th ed.,APHA, Washington, D.C. 4.Eaton A. D., Clesceri L. S. and Greenberg A W.,(Eds.), 2005, Standard Methods for the Examination of Water andWastewater, 21st ed., APHA, Washington, D.C. 5.Pollock M.R. and Knor R., 1943, Biochem J., 37:476. 6.MacFaddin J., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria., Vol. 1, Williams andWilkins, Baltimore. |
| Customized Product Available | No |
Write Your Own Review
Upsell Products
Tetrathionate Broth
As low as
₹1,800.00