Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Ready Prepared Plates#4fa25a
Intended Use
Recommended for isolation of Candida species (appears as white coloured) from clinical alposition**
Composition
| Ingredients | g/L |
|---|---|
| Peptonized SM powder | 25.000 |
| Malt extract | 10.000 |
| Dextrose (Glucose) | 15.000 |
| Selective mixture | 0.500 |
| Agar | 15.000 |
| Can Selective Supplement, Modified (FD824) | 1 vial |
| Selective agent | 450mg |
| pH after sterilization( at 25°C) | 5.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Candidiasis is a fungal infection caused by a yeast (a type of fungus) called Candida. White fungus causes an infection in the lungs similar to COVID-19. Candida species responsible for the infection are Candida albicans and Candida auris.ilt usually affects nose, mouth, lungs and stomach or nail beds. People suffering from COVID-19, HIV/AIDS and other viral diseases, congenital bone marrow disease, cancers and untreated or irregularly treated diabetes have reduced immunity and are prone to aquire the infection. This medium is specifically designed to promote rapid and selective growth of Candida strains. Peptonized SM powder and malt extract serves as a source of nitrogeneous and carbonaceous compounds, long chain amino acids, vitamins and other essential nutrients. Dextrose in the medium serves as a rich source of energy. Selective mixture suppresses the growth of mycelial fungi and inhibits bacterial flora.
Type of specimen
Clinical samples: eye lesion, nasal swabs, other site of infections etc.
Specimen Collection and Handling
Specimens from the eye, nose, nasopharynx and other sites of infection are usually collected with the help of sterile swab and transported to the lab by using HiFungal™™ Transport medium w/swab (MS5478) which contains the transport medium along with the swab. Specimen collection should be carried out by trained personnel. After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro Diagnostic use only. For professional use only. Read the label before opening the pack. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling clinical specimens and culture. Standard guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual fungi differ in their growth requirement and therefore show variable growth patterns on the medium.
- Further biochemical and serological studies are to be carried out for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Candida Selective Agar in 90 mm disposable plates with smooth surface and absence of black particles/cracks/ bubbles
Colour of medium
Light yellow to amber coloured medium
Quantity of medium
25 ml of medium in 90 mm disposable plates.
pH
5.40-5.80
Sterility Check
Passes release criteria
Growth Promotion Test
Cultural characteristics observed after an incubation at 25-30°C for 24-48 hours.
Cultural Response
| Organism | Growth |
|---|---|
| Candida albicans ATCC 10231 (00054*) | luxuriant |
| Candida auris CDC B 11903 | luxuriant |
| Mucor racemosus ATCC 42647 | none-poor |
| #Aspergillus brasiliensis ATCC 16404 (00053*) | none-poor |
| Escherichia coli ATCC 25922 (00013*) | inhibited |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | inhibited |
| Pseudomonas aeruginosa ATCC 27853 (00087*) | inhibited |
Key: (#) - Formerly known as Aspergillus niger, (*) - corresponding WDCM numbers
Storage and Shelf Life
On receipt store between 20-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,2).
| Product Name | Candida Selective Agar Plate |
|---|---|
| SKU | MP5477 |
| Customized Product Available | No |
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