Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Solid Media in Glass Bottles#4fa25a
Intended Use
Recommended for antibiotic plate assay of colistimethate, Colistin and polymyxin B in accordance with USP.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Tryptone # | 17.000 |
| Soya peptone ## | 3.000 |
| Dextrose (Glucose) | 2.500 |
| Sodium chloride | 5.000 |
| Dibasic potassium phosphate | 2.500 |
| Agar | 12.000 |
| Polysorbate 80 | 10.000 |
| pH after sterilization | 7.2±0.1 |
**Formula adjusted, standardized to suit performance parameters
# Pancreatic digest of casein
## Papaic digest of soybean
Directions
Antibiotic Assay Medium No. 10 is a ready to use solid media in glass bottle. The medium is pre-sterilized, hence it does not need sterilization. Medium in the bottle can be melted either by using a pre-heated water bath or any other method. Slightly loosen the cap before melting. When complete melting of medium is observed dispense the medium in tubes as butts /slants or in plates as desired and allow to solidify.
Principle And Interpretation
This medium is used as seed agar for assay of Polymyxin B, Colistimethate, Colistin. The medium composition is in accordance to USP and CFR (1,2) and numerically identical with the name assigned by Groove and Randall (3). Combination of tryptone and soya peptone provide essential nutrients for the growth of test organisms. Dextrose provides the carbon source, enhances the growth of test organism. Phosphates in the medium enhances buffering action and sodium chloride maintains osmotic equilibrium. Polymixins are reported to have slow diffusion in agar giving smaller zone of inhibition (4). Hence the reduced agar concentration (1.2%) in this medium improves the diffusion of polymixin in the medium. Polysorbate 80 is reported to function synergistically with polymixins on spheroplasts of Pseudomonas aeruginosa. Polysorbate 80 enhances the penetration of Polymyxin to the cytoplasmic membrane and hence is an appropriate ingredient in the medium used for assay of Polymyxin (5).
Type of specimen
Pharmaceutical preparations
Specimen Collection and Handling
Test organisms are inoculated in sterile seed agar pre-cooled to 40-45°C and spread evenly over the surface of solidified base agar. All conditions in the microbiological assay must be controlled carefully. The use of standard culture media in the test is one of the important steps for good results. After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Freshly prepared plates must be used or it may result in erroneous results.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Antibiotic Assay Medium No. 10 in glass bottle.
Colour of the medium
Medium amber coloured medium
pH
7.10-7.30
Quantity of medium
250 ml of medium in glass bottle.
Growth Promotion Test
As per United States Pharmacopoeia
Sterility Check
Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 32-35°C for 24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Antibiotics assayed |
|---|---|---|---|---|
| Bordetella bronchiseptica ATCC 4617 | 50-100 | luxuriant | >=70% | Colistimethate, Colistin, Polymyxin B |
| Pseudomonas aeruginosa ATCC 25619 | 50-100 | luxuriant | >=70% | Carbenicillin |
Storage and Shelf Life
On receipt store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).
| Product Name | Antibiotic Assay Medium No. 10 |
|---|---|
| SKU | SMU225CCL |
| Customized Product Available | No |
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