Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Intended use
For detection and enumeration of E. coli and coliforms.
Directions
The test sample should be filtered through a sterile membrane filter having pore size of 0.45µ. Rehydrate the nutrient pad with 2.0-2.5 ml sterile distilled / purified water. After filtration, remove the membrane filter aseptically using sterile forceps. Place the membrane filter on rehydrated nutrient pad. Incubate the inoculated nutrient. Interpret the results qualitatively by observing the presence or absence of growth and quantitatively by counting the number of colonies on the surface of the membrane filter and calculating CFU/ml.
Principle And Interpretation
Field of Application: Water (Standard TNV 75, 7837, 2002), food and other samples. DriFilter Membrane Nutrient Pad Medium is ready to use sterile culture media in the form of a 50 mm biological inert absorbent pads impregnated with M-Endo medium, then dried and sterilized in 55 mm petri plate. They eliminate the need of laborious media preparation and autoclaving procedures. The nutrient pads are to be just rewetted with sterile distilled water and are ready to use. Use of nutrient pads allows larger sample volumes to be tested at a time. Interpretation of results is directly by counting the CFUS and also quantifies the microbial load present in the sample. M-Endo medium was used for studying milk lines of milk handling equipment (1) and for examination of swimming pool (2) waters using membrane filter technique. This medium gives higher counts and is most satisfactory of the many media used, since coliform colonies develop rapidly (3), preliminary enrichment and saturated relative humidity are not necessary and results are in good agreement with the Standard Methods MPN Test.
Type of specimen
Water samples
Specimen Collection and Handling
For water samples follow appropriate techniques for handling specimens as per established guidelines (1,2). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Due to varying nutritional requirements, some strains may be encountered that grow poorly.
- If the inoculum is too heavy, the sheen may be suppressed.
- Occasionally, non coliform organisms may produce typical sheen colonies. Coliform organisms may also occasionally produce atypical colonies, including dark red or nucleated colonies without sheen.
- Further serological and biochemical testing is necessary for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Dry filter membrane pad of 50mm diameter
Colour
Purple coloured nutrient pad
Sterility check
Passes release criteria
Cultural response
Cultural characteristics observed after incubation at 35-37°C for 18-24 hours
| Organism | Growth | Colour of colony |
|---|---|---|
| Klebsiella pneumoniae ATCC 13883 (00097*) | Luxuriant | Pink,mucoid |
| Salmonella Typhimurium ATCC 14028 (00031*) | Luxuriant | Colourless |
| Escherichia coli ATCC 25922 (00013*) | Luxuriant | Pink with metallic sheen |
Key: (*) - Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 10-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | M-Endo Medium (without Membrane Filter) |
|---|---|
| SKU | MF010 |
| Customized Product Available | No |
Write Your Own Review