This kit provides a fast and easy method to purify total DNA from yeastsamplesfor downstream applications such as PCR, Southern blotting technique etc. The DNA purification procedure using the columns comprises of three steps viz. adsorption of DNA to the Super? S membrane, removal of residual contaminants and elution of pure genomic DNA. The Super? S columns have
a high binding capacity and high-quality genomic DNA is obtained from various species
This kit simplifies isolation of DNA from yeast by the vacuum based innovative Super? S column technology. Yeast cells (Saccharomyces cerevisiae, Candida albicans), are grown in log phase and spheroplasts are subsequently prepared. Following lysis, the DNA is purified using Super? S columns which contains specially developed membranes for optimal binding of genomic DNA. After the initial binding of DNA, impurities like proteins, polysaccharides, low molecular weight metabolites and salts are removed by short washing steps.