This kit provides a fastest and easiest way to purify viral DNA for reliable use in amplification technologies. Viral DNA can be purified from plasma (treated with anticoagulant EDTA), serum,and other cell?free body fluids. Samples may be fresh or frozen, but if frozen, should not be thawed more than once. Repeated freeze–thawing of plasma samples will lead to reduced viral
titers and should be avoided for optimal sensitivity. The Super? S columns have a high binding capacity and high-quality DNA is obtained from various samples.
This kit simplifies isolation of DNA from fresh, old (more than 24 hours) and frozen plasma,
serum and other body fluids. Viral DNA purification involves cell lysis, which is achieved by
incubation of sample in a solution containing chaotropic ions in the presence of Proteinase K at
56oC. When Carrier RNA is added to Elution Solution (RNase? Free Water), it improves the binding of viral DNA to the Super? S Column especially in the case of low?titer samples, andlimits possible degradation of the viral DNA due to any residual RNase activity. high-quality DNA is finally eluted in the Elution Buffer provided with the kit.