Insta NX® Food DNA Purification Kit provides a fast and easy method for purification of total DNA from various sources of solid and liquid food samples for downstream applications such as PCR, RT?PCR, Southern blotting technique etc.The DNA purification procedure using the column comprises of three steps viz. adsorption of DNA to the Super? S membrane, removal of residual contaminants and elution of pure genomic DNA. The Super? S columns have a high binding capacity and high-quality genomic DNA is obtained from various samples.
This kit simplifies isolation of DNA from variety of raw and processed food matrices. Genomic
DNA purification from food involves cell lysis, which is achieved by incubation of food in a
solution containing chaotropic ions in the presence of Proteinase K at 65°C. This kit simplifies
isolation of DNA from food samples with vacuum based innovative Super? S column technology.
The Super? S columns contains specially developed membranes for optimal binding of the DNA.
After the initial binding of DNA, impurities like proteins, polysaccharides, low molecular weight
metabolites and salts are removed by short washing steps. high-quality DNA is finally eluted in
the Elution Buffer.
The salient features of this kit are as follows:
a. Cross? Contamination free
b. 12 samples can be processed at a time.
c. Prefilled reagent cartridge
d. High purity, high yield, as good as manual.