Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for selective cultivation of Pseudomonas aeruginosa.
Composition**
| Ingredients | g / L |
|---|---|
| Peptone | 10.000 |
| HM peptone B# | 10.000 |
| Sodium chloride | 5.000 |
| Cetrimide | 0.300 |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract
Directions
Suspend 25.3 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes or flasks or as desired. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 45-50°C.
Principle And Interpretation
Pseudomonas aeruginosa grows well on all normal laboratory media. Cetrimide Broth Base is the modification of the formula designed by King, Ward and Raney (1) and is useful for the cultivation of P. aeruginosa. This medium is selective as it contains cetrimide (Cetyl trimethyl ammonium bromide), which inhibits other bacteria except P. aeruginosa. This medium is therefore, important in the identification of P. aeruginosa. Cetrimide Broth is used for the examination of cosmetics (2) and clinical specimens (3,4) for the presence of P. aeruginosa, as well as for evaluating the efficacy of disinfectants against this organism (5). Peptone and HM peptone B provide necessary nutrients for P. aeruginosa. Cetrimide acts as a quaternary ammonium, cationic detergent that causes release of nitrogen and phosphorus from bacterial cells other than Pseudomonas aeruginosa. Sodium chloride maintains osmotic equilibrium in the medium.
Type of specimen
Clinical samples - pus, skin samples, urine.
Specimen Collection and Handling:
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (3,4). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
In Vitro diagnostic Use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow coloured homogeneous free flowing powder
Colour and Clarity of prepared medium
Light amber coloured clear to slightly opalescent solution in tubes
Reaction
Reaction of 2.53% w/v aqueous solution at 25°C. pH: 7.2±0.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | >=104 | inhibited |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Cetrimide Broth |
|---|---|
| SKU | M862 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. King E.O., Ward M.K. and Raney D.E., 1954, J. Lab. Clin. Med., 44(2):301. |
| Customized Product Available | No |
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