Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for isolation, cultivation and identification of a wide variety of obligate anaerobic bacteria from clinical and non-clinical samples.
Composition
| Ingredients | g/L |
|---|---|
| Tryptone | 17.000 |
| Soya peptone | 3.000 |
| Dextrose (Glucose) | 6.000 |
| Sodium chloride | 2.500 |
| Sodium thioglycollate | 0.500 |
| L-Cystine | 0.250 |
| Sodium sulphite | 0.100 |
| Hemin | 0.005 |
| Vitamin K1 | 0.0001 |
| Agar | 0.700 |
| Sodium bicarbonate | 1.000 |
| Final pH (at 25°C) | 7.0±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 31.06 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at A118-121°C for 15 minutes. Aseptically add 10% rabbit or horse serum. Dispense in tubes or flasks as desired. Cool and dry under 85% N2,10% H2 and 5% CO2 atmosphere. A corresponds to at 12-15 lbs pressure respectively.
Principle And Interpretation
Enriched Thioglycollate Medium is recommended for use in isolation and cultivation of fastidious and obligate anaerobic bacteria from clinical materials (1). This medium is often used for susceptibility testing of anaerobes by broth disk elution method. This medium is the modification of original Brewers formulation (2,3), with the addition of vitamin K1, sodium bicarbonate, hemin and rabbit or horse serum. Tryptone and soya peptone supports growth of wide variety of fastidious microorganisms. Sodium thioglycollate lowers the oxidation-reduction potential for anaerobic growth and also neutralizes the bacteriostatic effect of mercurial compounds. Most organisms show earlier and more vigorous growth in presence of dextrose, hemin and vitamin K1. Hemin is the source of X-factor, which stimulates the growth of many microorganisms.
Type of specimen
Clinical : wound swabs, skin swabs or scrapings, tooth tartar etc.
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- The tubes should not be reheated as frequent boiling leads to development of toxic products.
- Prior to use the medium should be boiled once to remove the absorbed oxygen.
- Before inoculation, the tubes should be brought to room temperature
- The medium should not be used in fermentation process as medium contains yeast extract which is high in carbohydrate content.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium: Light amber coloured, clear to slightly opalescent solution in tubes
Reaction: Reaction of 3.0% w/v aqueous solution at 25°C. pH: 7.0±0.2
pH: 6.80-7.20
Cultural Response
Cultural characteristics observed under anaerobic condition, after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Bacteroides vulgatus ATCC 8482 | 50-100 | luxuriant |
| Clostridium perfringens ATCC 12924 | 50-100 | luxuriant |
| Clostridium sporogenes ATCC 11437 | 50-100 | luxuriant |
| Neisseria meningitidis ATCC 13090 | 50-100 | luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant |
Storage and Shelf Life
Store between 10-30°C in tightly closed container and the prepared medium at 15-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | Enriched Thioglycollate Broth |
|---|---|
| SKU | M738 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Allen S. D., Siders J. A. and Movler M., 1985, In Manual of Clinical Microbiology, Lennette, Balows, Hausler and Shadomy(Eds.), 4th Ed., ASM, Washington, D.C. 2.Brewer J. H., 1940 and 1943, J. Bacteriol., 39:10 and 46:395. 3.Brewer J. H., 194 3.J. Bacteriol., 46:395. |
| Customized Product Available | No |
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