Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition
| Ingredients | Gms/Litre |
|---|---|
| Beef muscles | 454.000 |
| Calcium carbonate | 5.000 |
| Egg white from (eggs) | 6.000 |
| Final pH (at 25°C) | 7.2±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 15 grams in 100 ml distilled water. Allow to stand with frequent agitation for thorough wetting. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Note: Due to presence of Calcium carbonate, the prepared medium forms opalescent solution with white precipitate.
Principle And Interpretation
Organisms that are able to grow in the absence of oxygen are termed as anaerobic organisms. Depending upon their absolute requirement for an on-oxygen environment, they are either classified as facultative or obligate. Egg Meat Medium was used by Rettger for his studies on Escherichia coli and Enterobacter aerogenes (1). This medium contains particles of beef muscles, calcium carbonate and egg white. This medium was later used in studies of intestinal putrefaction (2). Egg Meat Medium was subsequently used in the study of spore-forming anaerobes including Clostridium perfringens (3, 4). This medium is also recommended by AOAC for maintenance of Clostridium used in testing of sporicidal activity of liquid and gaseous chemicals (5).
Beef muscle serves as sources of carbon, nitrogen, salts, vitamins and other nutrients required to support bacterial growth. Egg white serves a source of protein for the determination of proteolytic activity. Calcium carbonate helps to neutralize the acids and also helps in creating anaerobic atmosphere by displacing the oxygen in the medium.
Refer standard procedures for inoculation (5).
Quality Control
Appearance
Light brown to brown homogeneous free flowing powder
Colour and Clarity of prepared medium
Light amber coloured, clear to slightly opalescent solution with white precipitate over brown coloured meat granules.
Reaction
Reaction of 15% w/v aqueous solution at 25°C. pH : 7.2±0.2
pH
7.00-7.40
Cultural Response
M735: Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Clostridium perfringens ATCC 12924 | 50-100 | luxuriant |
| Clostridium sporogenes ATCC 11437 | 50-100 | luxuriant |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Egg Meat Medium (Revised as Egg M Medium) |
|---|---|
| SKU | M735 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Rettger L. F., 1906, J. Biol. Chem., 2:71. 2.Rettger L. F., 190 3.Am. J. Physiol., 8:284. 3.Reddish G. F. and Rettger L. F., 1924, J. Bacteriol., 9:13. 4.Reddish G. F. and Rettger L. F., 1923, J. Bacteriol., 8:375. 5.Horwitz, (Ed.), 2000, Official Methods of Analysis of AOAC International, 17th Ed., Vol. I, 6. 3.05A (a) (4), AOACInternational, Gaithersburg, Md. |
| Customized Product Available | No |
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