Modified AEA Sporulation Medium Base is used for early sporulation of Clostridium perfringens from food. This medium is recommended by APHA and is a modification of the original medium of Taniguti and is recommended for the early sporulation of C. perfringens from foods. C. perfringens ferments raffinose to produce acid. To test for acid, transfer 1 ml of culture to a test tube or spot plate and add 2 drops of 0.0 bromothymol blue. A yellow colour indicates acid production.
1. Collee J. G., Fraser A. G., Marmion B. P., Simmons A., (Eds.), Mackie and McCartney, Practical Medical Microbiology,1996, 14th Edition, Churchill Livingstone.2.Harmon S. M., and Kautter D. A., 1986, J. Food Prot. 49:706.3.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., APHA, Washington, D.C.4.Taniguti T., 1969, J. Food Hyg. Soc. Jpn. 9: 219.