Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Sea Water#CC293D
Intended Use
Recommended for detection of saccharolytic marine bacteria.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| M-protein powder | 1.000 |
| Starch | 10.000 |
| Sea water | 37.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
# - Equivalent to Casein powder
Directions
Suspend 63.0 grams in purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Starch M-Protein Agar is recommended for detection of saccharolytic marine bacteria and Actinomycetes (1,7). Widely distributed in nature, Actinomycetes constitute a considerable proportion of the population of soil, lakes and river muds. Traditionally Actinomycetes have been isolated from terrestrial sources, although the first report of mycelium forming Actinomycetes being recovered from marine sediments appeared several decades ago (8). Marine sediments are known potential sources for isolation of novel actinomycetes yielding new products and are recognized as source of novel antibiotic and anticancer agents (2,4). Actinomyces have an extensive impact on the environment by decomposing and transforming a wide variety of complex organic residues. Actinomycetes thus represent an important group of microbes found in environment and plays significant role not only in therapeutic applications but also on recycling of organic matter (6). This medium has starch as the complex carbohydrate source and M-protein powder as nitrogen source. The salts of seawater provides complex ionic sources that makes the medium suitable for marine microbial flora and also buffers the medium.
Type of specimen
Marine isolates
Specimen Collection and Handling
For marine samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards. After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Due to nutritional variations, some strains may show poor growth on media.
- Further biochemical tests must be carried out for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Off-white to yellow coloured homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Yellow coloured clear to slightly opalescent gel forms in petri plates.
Reaction
Reaction of 6.3% aqueous solution at 25°C. pH: 7.2±0.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Streptococcus limosus ATCC 19778 | 50-100 | luxuriant | >=50% |
| Streptomyces praecox ATCC 3374 | 50-100 | luxuriant | >=50% |
| Vibrio cholerae ATCC 15748 | 50-100 | good-luxuriant | >=50% |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,5).
| Product Name | Starch Casein Agar (Revised as Starch M-Protein Agar) |
|---|---|
| SKU | M801 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Wellington, E.M.H. Cross, T.(1983)- Taxonomy of antibiotic producing Actinomycetes and new approaches to their selectiveisolation. In: "Progress in industrial microbiology?" Bushell, M. E. (eds.). Elsevier, Amsterdam. pp. 36.2.CRC Handbook Series in Nutrition and Food, 1987 Section G: Diets, Culture Media, Food Supplements- Vol III. CultureMedia for Microorganisms and plants by Miloslav Rechcigl,Jr.3.Weyland, H. (1969). Actinomycetes in North Sea and Atlantic Ocean sediments. Nature 223, 858.4.Jensen, P.R., Dwight, R. and Finical, W. (1991). Distribution of Actinomycetes in near shore tropical marine sediments,Journal of Applied Environmental Microbiology 57, 1102-1108.5.Goodfellow M. and Haynes, J.A (1984). Actinomycetes in marine sediments. In:Biological, Biochemical and BiomedicalAspects of Actinomycetes. Oritz-Oritz, L., Bojali, C.F. and Yakoleff, V. (eds.). Academic Press. New York, London, pp.453-463.6.Srinivasan, M.C., Laxman R.S. and Deshpande M.V.(1991). Physiology and nutrition aspects of actinomycetes- An overview.World journal of Microbial and Biotechnology 7,171-184.7.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition. |
| Customized Product Available | No |
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Actinomyces Agar
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