Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Enrichment#CC293D
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Peptic digest of animal tissue | 20.000 |
| Sodium chloride | 30.000 |
| Final pH (at 25°C) | 8.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 50 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Clinical materials containing small numbers of Vibrio should be inoculated into an enrichment medium prior to plating onto a selective medium, such as TCBS Agar (M189). Alkaline Peptone Water is a suitable enrichment broth for this purpose (1-3). This medium is recommended by APHA (4) for enrichment of Vibrio species from seafood, infectious materials and other clinical specimens such as faeces (5). A slight modification of this medium has recently been approved by the ISO Committee (6) for detection of Vibrio species.
Peptic digest of animal tissue provides amino acids and other nitrogenous substances. Sodium chloride maintains osmotic equilibrium.
Add 10 grams of seafood to 90 ml of Alkaline Peptone Water and incubate for upto 18-20 hours at 37°C. Prolonged incubation will result in growth of the suppressed contaminating organisms to develop (7). Growth in tubes is indicated by turbidity compared to an un-inoculated tube (control). Growth from the enrichment broth is used for plating on selective media. For biochemical identification a pure culture is recommended.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light yellow coloured clear solution without any precipitate
Reaction
Reaction of 5% w/v aqueous solution at 25°C. pH: 8.6±0.2
pH
8.40-8.80
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
Cultural Response
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Vibrio cholerae ATCC 15748 | 50-100 | luxuriant |
| Vibrio parahaemolyticus ATCC 17802 | 50-100 | luxuriant |
Storage and Shelf Life
Store below 30°C in tightly closed container and prepared medium at 2-8°C. Use before expiry date on the label.
| Product Name | Alkaline Peptone Water |
|---|---|
| SKU | M618I |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Gilligan, Janda, Karmali and Miller, 1992, Cumitech 12A, Laboratory Diagnosis of Bacterial Diarrhea, Coord. Ed., Nolte,American Society for Microbiology, Washington, D.C. 2.Forbes B. A., Sahm A. S., and Weissfeld D. F., Bailey & Scotts Diagnostic Microbiology, 10th Ed., 1998, Mosby, Inc.,St. Louis, Mo. 3.Isenberg, (Ed.), 1992, Clinical Microbiology Procedures Handbook, Vol. I, American Society for Microbiology, Washington,D.C. 4.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,APHA, Washington, D.C. 5.Cruikshank R., 1968, Medical Microbiol., 11th Ed., Livingstone Ltd., London. 6.International Organization for Standardization (ISO), 1990, Draft ISO/DIS 8914. 7.Finegold S. M. and Martin W. J., 1982, W. J. Bailey and Scotts Diagnostic Microbiol, 6th Ed., C.V. Mosby Co., St. Louis,p. 242. |
| Customized Product Available | No |
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