Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Ready Prepared Collection and Transport Media#CC293D
Intended use
Recommended for recovery of aerobic, anaerobic and fastidious bacteria from faecal specimens.
Composition**
| Ingredients | g/L |
|---|---|
| Disodium hydrogen phosphate | 1.100 |
| Sodium thioglycollate | 1.500 |
| Sodium chloride | 5.000 |
| Agar | 5.000 |
| Final pH (at 25°C) | 8.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Collect sample or specimen using capped swab. Discard cap of the tube with medium and and insert the capped swab with the sample till the bottom of the medium. Tighten the cap firmly. Process the sample as soon as possible to obtain optimum recovery. In case where immediate recovery (i.e within 2 hours) is not possible, specimens can be stored at 2-25°C and processed within 48 hours.
Principle And Interpretation
Proper collection and transportation of faecal specimens is vital for detection of faecal pathogens. Cary and Blair (1) devised this medium to provide conditions that will allow and increase survival of organisms without aiding multiplication due to minimal nutrients. Sodium thioglycollate in the medium provides a low oxidation reduction potential. An alkaline pH of the medium prevents bacterial destruction due to formation of acid. Sterile cotton swabs allow absorption of specimen material while polystyrene shaft allows semi flexibility to the swab stick, aiding in collection.
Type of specimen
Clinical samples- Faeces
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (1,2). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro Diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Users are recommended to validate the medium for any specific microorganisms other than mentioned in the certificate of analysis based on users unique requirement, as each lot has been tested for the organisms specified on the certificate of analysis.
- Some growth of accompanying contaminants may also occur during longer period of transit.
- Biochemical characterization is required on colonies of pure culture for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Cary-Blair medium in tubes with sterile viscous swabs.
Colour
Light amber coloured medium
Quantity of Medium
8ml of medium in tubes
Cultural response
Viability of following organisms was established for a period of 48 hours. Organisms grew luxuriantly when recovered on Tryptone Soya Agar (M290) and incubated at 35 - 37°C for 18-24 hours.
Sterility Check
Passes release criteria
Cultural Response
| Organism | Recovery |
|---|---|
| # Klebsiella aerogenes ATCC 13048 (00175*) | Good - Luxuriant |
| Escherichia coli ATCC 25922 (00013*) | Good - Luxuriant |
| Klebsiella pneumoniae ATCC 13883 (00097*) | Good - Luxuriant |
| Neisseria meningitidis ATCC 13090 | Good - Luxuriant |
| Salmonella Typhimurium ATCC 14028 (00031*) | Good - Luxuriant |
| Shigella flexneri ATCC 12022 (00126*) | Good - Luxuriant |
| Vibrio parahaemolyticus ATCC 11344 | Good - Luxuriant |
| Vibrio cholerae ATCC 15748 | Good - Luxuriant |
Key: (*) Corresponding WDCM numbers. (#) Formerly known as Enterobacter aerogenes
Storage and Shelf Life
Store between 5-30°C with caps firmly screwed. DO NOT FREEZE. Use before expiry date on label.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | HiCulture™ Transport Swabs w/ Cary Blair Medium |
|---|---|
| SKU | MS202 |
| Customized Product Available | No |
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