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HiCulture™ Transport Swabs w/ Alternative Thioglycollate Medium
Ready Prepared Collection and Transport Media#CC293D
Intended Use
Recommended for transportation of aerobes, anaerobes and microaerophiles.
Composition**
| Ingredients | Gms/Litre |
|---|---|
| Pancreatic digest of casein | 15.000 |
| Yeast extract | 5.000 |
| Dextrose (Glucose) | 5.500 |
| Sodium chloride | 2.500 |
| L-Cystine | 0.500 |
| Sodium thioglycollate | 0.500 |
**Formula adjusted, standardized to suit performance parameters
Directions
Remove cap from the tube. Collect sample using capped swab. Discard cap on tube, replace with capped swab.
Principle And Interpretation
Alternative Thioglycollate Medium contains sodium thioglycollate that can neutralize the bacteriostatic effect of mercurial preservatives. Absence of agar makes it suitable for testing viscous materials and devices having tubes with small lumina. Casein enzymic hydrolysate, yeast extract, dextrose, L-cystine provides nitrogenous and carbonaceous compounds, vitamin B complex, trace elements and other essential growth nutrients. The composition matches with the medium recommended in pharmacopoeias (1, 2,3). Sterile cotton swabs allow absorption of specimen material while polystyrene shaft allows semiflexibility to the swab stick, aiding in collection.
Quality Control
Appearance: Sterile Alternative Thioglycollate Medium in tubes with sterile cotton swabs.
Colour: Yellow coloured medium
Quantity of Medium: 8ml of medium in tubes
Cultural response: Viability of following organisms was established for a period of 48 hours. Organisms grew luxuriantly when recovered on Tryptone Soya Agar (M290) and incubated at 35 - 37°C for 18-24 hours.
Sterility test: Passes release criteria
Cultural Response
| Organism | Recovery |
|---|---|
| Bacteroides vulgatus ATCC 8482* | Luxuriant |
| Clostridium sporogenes ATCC 11437* | Luxuriant |
| Candida albicans ATCC 10231** | Luxuriant |
| Bacillus subtilis ATCC 6633 | Luxuriant |
| Bacteroides fragilis ATCC 25285* | Luxuriant |
| Micrococcus luteus ATCC 10240 | Luxuriant |
| Neisseria meningitidis ATCC 13090 | Luxuriant |
| Staphylococcus aureus ATCC 25923 | Luxuriant |
| Streptococcus pyogenes ATCC 19615 | Luxuriant |
Storage and Shelf Life
Store between 5 – 25°C with caps firmly screwed. DO NOT FREEZE. Use before expiry date on label.
| Product Name | HiCulture™ Transport Swabs w/ Alternative Thioglycollate Medium |
|---|---|
| SKU | MS010 |
| Customized Product Available | No |

